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Titolo:
Relative and absolute changes of activated platelets, microparticles and platelet aggregates after activation in vitro
Autore:
Matzdorff, AC; Berchner, D; Kuhnel, G; Kemkes-Matthes, B; Pralle, H; Voss, R;
Indirizzi:
Univ Giessen, Dept Internal Med, Div Haematol Oncol, D-35385 Giessen, Germany Univ Giessen Giessen Germany D-35385 tol Oncol, D-35385 Giessen, Germany Univ Giessen, Div Cardiol, D-35385 Giessen, Germany Univ Giessen GiessenGermany D-35385 v Cardiol, D-35385 Giessen, Germany
Titolo Testata:
HAEMOSTASIS
fascicolo: 6, volume: 28, anno: 1998,
pagine: 277 - 288
SICI:
0301-0147(199811/12)28:6<277:RAACOA>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
THROMBOTIC THROMBOCYTOPENIC PURPURA; FLOW CYTOMETRIC DETECTION; MICROVESICLES; ANGIOPLASTY; DISEASE;
Keywords:
flow cytometry; CD62p; microparticles; platelet aggregates;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Matzdorff, AC Univ Giessen, Dept Internal Med, Div Haematol Oncol, Klin Str 36, D-35385 Giessen, Germany Univ Giessen Klin Str 36 Giessen Germany D-35385 n, Germany
Citazione:
A.C. Matzdorff et al., "Relative and absolute changes of activated platelets, microparticles and platelet aggregates after activation in vitro", HAEMOSTASIS, 28(6), 1998, pp. 277-288

Abstract

Standard flow cytometers provide relative numbers of activated platelets, microparticles, and platelet aggregates. With fluorescent beads it is now possible to determine absolute numbers. Whole blood and platelet-rich plasmawere incubated with agonists (ADP, collagen, thrombin). CD62p expression, microparticle and platelet aggregate formation were measured. Flow-Count Fluorospheres(R) were added to calculate absolute concentrations. After activation there was an increase in the percentage of CD62p-positive platelets. However, the total number of platelets decreased and therefore the absolutenumber of CD62p-positive platelets did not increase but decreased. The number of CD62p-positive platelets decreased not as much as the number of CD62p-negative platelets, which explains why the relative percentage of CD62p-positive platelets increased. A similar increase in percent and decrease in absolute counts was found for microparticles. Platelet aggregates increasedboth in relative and absolute numbers, These results suggest that the detection of activated platelets by flow cytometry has to be complemented by the determination of the absolute concentrations to avoid misinterpretation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/04/20 alle ore 23:09:34