Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Chemical forms of technetium in tomato plants; TcO4-, Tc-cysteine, Tc-glutathione and Tc-proteins
Autore:
Krijger, GC; Harms, AV; Leen, R; Verburg, TG; Wolterbeek, B;
Indirizzi:
Delft Univ Technol, Interfac Reactor Inst, Dept Radiochem, NL-2629 JB Delft, Netherlands Delft Univ Technol Delft Netherlands NL-2629 JB 29 JB Delft, Netherlands
Titolo Testata:
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
fascicolo: 1, volume: 42, anno: 1999,
pagine: 69 - 81
SICI:
0098-8472(199908)42:1<69:CFOTIT>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
TC-99; SOIL; BEHAVIOR;
Keywords:
artefacts; compartment analysis; Lycopersicon esculentum; technetium-speciation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Krijger, GC Delft Univ Technol, Interfac Reactor Inst, Dept Radiochem, Mekelweg 15, NL-2629 JB Delft, Netherlands Delft Univ Technol Mekelweg 15 Delft Netherlands NL-2629 JB s
Citazione:
G.C. Krijger et al., "Chemical forms of technetium in tomato plants; TcO4-, Tc-cysteine, Tc-glutathione and Tc-proteins", ENVIR EXP B, 42(1), 1999, pp. 69-81

Abstract

The rapid accumulation of Tc, originating from nuclear power generation, in plants and the phytotoxicity at low Tc levels suggest an interaction between Tc and proteins. In the present study, different chemical forms of Tc in tomato plants were identified using an approach explicitly designed to prevent artefacts. After precipitation of Tc-proteins (80% ammonium sulphate), TcO4-, Tc-cysteine, and Tc-glutathione were determined, using reversed-phase ion-pair chromatography. A vast range of Tc-proteins was detected usinga gradient of ammonium sulphate precipitation or hydrophobic interaction chromatography. The hypothesis that Tc, as an S-analogue, is organo-metallically incorporated into organic substances appears to be rather unlikely, because all reduced Tc in the leaves was susceptible to ligand-exchange with 2-mercaptoethanol. A ligand exchange route for reduced Tc from proteins to cysteine and glutathione in the leaves was suggested, using Tc species ratios and mathematical modelling. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/01/20 alle ore 17:39:52