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Titolo:
C-erbB-2 expression and its relationship with ploidy, p53 abnormalities and epidermal growth factor receptor content in human non-small cell lung cancer
Autore:
Lopez-Guerrero, JA; Bolufer-Gilabert, P; Vera-Sempere, FJ; de la Concha, IM; Barragan-Gonzalez, E;
Indirizzi:
Univ Hosp La Fe, Dept Clin Biopathol, Mol Biol Lab, Valencia 46009, Spain Univ Hosp La Fe Valencia Spain 46009 Mol Biol Lab, Valencia 46009, Spain Univ Hosp La Fe, Serv Pathol, Valencia 46009, Spain Univ Hosp La Fe Valencia Spain 46009 Serv Pathol, Valencia 46009, Spain Clin Univ Hosp, Serv Oncohemathol, Valencia 46010, Spain Clin Univ Hosp Valencia Spain 46010 Oncohemathol, Valencia 46010, Spain
Titolo Testata:
CLINICA CHIMICA ACTA
fascicolo: 1-2, volume: 285, anno: 1999,
pagine: 105 - 120
SICI:
0009-8981(199907)285:1-2<105:CEAIRW>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
HER-2/NEU GENE-EXPRESSION; INTRINSIC CHEMORESISTANCE; EGF-R; K-RAS; MUTATIONS; TUMOR; PROGNOSIS; PROTEIN; LINES; ACTIVATION;
Keywords:
non-small cell lung cancer; c-erbB-2 expression; p53 mutations; flow cytometry; p53 overexpression; epidermal growth factor receptor; recurrence;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Bolufer-Gilabert, P Univ Hosp La Fe, Dept Clin Biopathol, Mol Biol Lab, Avda Campanar 21, Valencia 46009, Spain Univ Hosp La Fe Avda Campanar 21 Valencia Spain 46009
Citazione:
J.A. Lopez-Guerrero et al., "C-erbB-2 expression and its relationship with ploidy, p53 abnormalities and epidermal growth factor receptor content in human non-small cell lung cancer", CLIN CHIM A, 285(1-2), 1999, pp. 105-120

Abstract

This study attempts to clarify the role of c-erbB-2 overexpression in human non-small cell lung cancer (NSCLC) and relate it with the p53 alterations, DNA index (D.I.) and epidermal growth factor receptor (EGFR) content in sixty four patients with NSCLC. c-erbB-2 and EGFR quantification were carried out from tissue homogenates using quantitative ELISA procedures. p53 alterations were determined by immunohistochemical (IHC) detection with the monoclonal antibody DO-7 and analysis for p53 mutations on exons 4 to 8 by single strand conformation polymorphism (SSCP). The D.I. was performed by flowcytometry. c-erbB-2 hyperexpression was found in 13 of 58 LC (22%), and itwas closely associated with hyperdiploid tumors (D.I. > 1.3; P=0.00). The p53 abnormalities detected by SSCP were statistically more frequent in hyperdiploid tumors (16/25; P = 0.015) than in diploid ones (8/30). No relationship between the results of IHC p53 and SSCP was found. The patients with c-erbB-2 hyperexpressing tumors were prone to have frequent relapses (P = 0.03), although the patients with hyperdiploid NSCLC are the ones with the highest relapse rate (P=0.008). From the results obtained in this study the following conclusions can be drawn: (a) c-erbB-2 hyperexpressing NSCLC are associated with abnormalities in other biological markers and with a greaterrate of relapses; (b) SSCP seemed to be more specific that LHC to detect p53 molecular abnormalities; and (c) the D.I. is the parameter more tightly related with relapse. (C) 1999 Elsevier Science B.V. All rights reserved.

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Documento generato il 01/12/20 alle ore 07:46:38