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Titolo:
Unusual self-association properties of transthyretin Y114C related to familial amyloidotic polyneuropathy: Effects on detection and quantification
Autore:
Ando, Y; Almeida, MD; Ohlsson, PI; Ando, E; Negi, A; Suhr, O; Terazaki, H; Obayashi, K; Ando, M; Saraiva, MJM;
Indirizzi:
Kumamoto Univ, Sch Med, Dept Internal Med 1, Kumamoto 860, Japan Kumamoto Univ Kumamoto Japan 860 ept Internal Med 1, Kumamoto 860, Japan Kumamoto Univ, Sch Med, Dept Ophthalmol, Kumamoto 860, Japan Kumamoto Univ Kumamoto Japan 860 d, Dept Ophthalmol, Kumamoto 860, Japan Umea Univ, Dept Med Biochem & Biophys, S-90185 Umea, Sweden Umea Univ Umea Sweden S-90185 ed Biochem & Biophys, S-90185 Umea, Sweden Umea Univ, Dept Internal Med 1, S-90185 Umea, Sweden Umea Univ Umea Sweden S-90185 Dept Internal Med 1, S-90185 Umea, Sweden Univ Porto, Inst Biol Mol & Celular, Amyloid Unit, P-4100 Porto, Portugal Univ Porto Porto Portugal P-4100 r, Amyloid Unit, P-4100 Porto, Portugal Univ Porto, Inst Ciencias Biomed Abel Salazar, P-4100 Porto, Portugal UnivPorto Porto Portugal P-4100 ed Abel Salazar, P-4100 Porto, Portugal
Titolo Testata:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
fascicolo: 2, volume: 261, anno: 1999,
pagine: 264 - 269
SICI:
0006-291X(19990802)261:2<264:USPOTY>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
PORTUGUESE TYPE; JAPANESE TYPE; TTR VARIANTS; PRE-ALBUMIN; GENE; PREALBUMIN; FIBRILS; MUTANT;
Keywords:
FAP; transthyretin; amyloid; mass spectrometry; Y114C TTR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Ando, Y Kumamoto Univ, Sch Med, Dept Internal Med 1, 1-1-1 Honjo, Kumamoto860, Japan Kumamoto Univ 1-1-1 Honjo Kumamoto Japan 860 Kumamoto 860, Japan
Citazione:
Y. Ando et al., "Unusual self-association properties of transthyretin Y114C related to familial amyloidotic polyneuropathy: Effects on detection and quantification", BIOC BIOP R, 261(2), 1999, pp. 264-269

Abstract

We performed biochemical and immunological examinations of heterozygotic carriers of the transthyretin (TTR) mutant Y114C associated with familial amyloidotic polyneuropathy (FAP), The total serum TTR levels in Y114C TTR carriers were extremely low when analyzed by single radial immunodiffusion (SRID), whereas by indirect enzyme-linked immunosorbent assay (ELISA) procedure, their total TTR concentrations were increased. Recombinant homozygotic Y114C TTR showed no immunoreactivity towards a TTR antibody when analyzed bySRID, whereas by the ELISA procedure presented the same degree of reactivity as that of normal TTR or isolated serum heterozygotic Y114C TTR. These results indicate that immunodifusion based techniques cannot properly determine TTR serum levels in Y114C carriers. Analyses of serum TTR of the Y114C TTR carriers by electrospray ionization mass spectrometry (ESI-MS) with theorifice corn voltage at 60 V revealed a small peak of the free Y114C TTR in addition to large TTR peaks of normal TTR, The levels of the free mutant TTR increased with the orifice corn voltage at 90 V. In contrast, increase in orifice voltage from 60 to 90 V produced a reduction in the level of normal TTR. The results suggest a different pattern of association between monomers in Y114C relative to normal TTR. (C) 1999 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 15:43:28