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Titolo:
hnRNP A2 and hnRNP L bind the 3 ' UTR of glucose transporter 1 mRNA and exist as a complex in vivo
Autore:
Hamilton, BJ; Nichols, RC; Tsukamoto, H; Boado, RJ; Pardridge, WM; Rigby, WFC;
Indirizzi:
Dartmouth Coll, Sch Med, Dept Med, Lebanon, NH 03756 USA Dartmouth Coll Lebanon NH USA 03756 Med, Dept Med, Lebanon, NH 03756 USA Dartmouth Coll, Sch Med, Dept Microbiol, Lebanon, NH 03756 USA Dartmouth Coll Lebanon NH USA 03756 Dept Microbiol, Lebanon, NH 03756 USA Vet Adm Med Ctr, White River Junction, VT 05009 USA Vet Adm Med Ctr WhiteRiver Junction VT USA 05009 Junction, VT 05009 USA Univ Calif Los Angeles, Sch Med, Dept Med, Los Angeles, CA 90095 USA Univ Calif Los Angeles Los Angeles CA USA 90095 Los Angeles, CA 90095 USA Univ Calif Los Angeles, Sch Med, Inst Brain Res, Los Angeles, CA 90095 USAUniv Calif Los Angeles Los Angeles CA USA 90095 Los Angeles, CA 90095 USA
Titolo Testata:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
fascicolo: 3, volume: 261, anno: 1999,
pagine: 646 - 651
SICI:
0006-291X(19990811)261:3<646:HAAHLB>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN BRAIN-TUMORS; GLUT1 MESSENGER-RNA; 3'-UNTRANSLATED REGION; GENE-EXPRESSION; LUNG-CANCER; PROTEINS; TRANSCRIPTION; CELLS; HEMANGIOBLASTOMA; PURIFICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Hamilton, BJ Dartmouth Coll, Hitchcock Med Ctr, Dept Med, Hanover, NH 03756 USA Dartmouth Coll Hanover NH USA 03756 d, Hanover, NH 03756 USA
Citazione:
B.J. Hamilton et al., "hnRNP A2 and hnRNP L bind the 3 ' UTR of glucose transporter 1 mRNA and exist as a complex in vivo", BIOC BIOP R, 261(3), 1999, pp. 646-651

Abstract

Recent work identified an RNA binding protein whose presence in brain tumors correlated with translational repression of Glut1 expression. RNase T1 mapping demonstrated that this protein bound an AU-rich response element (AURE) in the Glut1 3'UTR. Facilitated by its differential expression in braintumor cytosols, we identified this Glut1 RNA binding protein as hnRNP A2. Studies further demonstrated that hnRNP A2 was the major Glut1 RNA binding activity in other cell lines. Recombinant hnRNP A2 exhibited equivalent Glut1 RNA binding specificity, quite distinct from the related AURE binding protein hnRNP Al. These data indicate that hnRNP A2 is the Glut1 AURE bindingprotein whose cytoplasmic expression in gliomas is associated with translational repression and mRNA instability, Using this approach, we also identified the other major Glut1 3'UTR RNA binding activity as hnRNP L. Stimuli (hypoxia and hypoglycemia) which increase Glut1 mRNA stability selectively decreased polysomal levels of hnRNP A2 and L,. Immunoprecipitation demonstrated that hnRNP A2 and L, exist as a complex in vivo. As a result of these and other studies, we conclude that hnRNP A2 and L associate in vivo and independently bind the 3'UTR of Glut1 mRNA. (C) 1999 Academic Press.

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Documento generato il 06/07/20 alle ore 08:32:50