Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Effect of pregnancy, postnatal growth, and gender on renal sulfate transport
Autore:
Lee, HJ; Balasubramanian, SV; Morris, ME;
Indirizzi:
SUNY Buffalo, Sch Pharm, Dept Pharmaceut, Buffalo, NY 14260 USA SUNY Buffalo Buffalo NY USA 14260 Dept Pharmaceut, Buffalo, NY 14260 USA
Titolo Testata:
PROCEEDINGS OF THE SOCIETY FOR EXPERIMENTAL BIOLOGY AND MEDICINE
fascicolo: 4, volume: 221, anno: 1999,
pagine: 336 - 344
SICI:
0037-9727(199909)221:4<336:EOPPGA>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
BORDER MEMBRANE-VESICLES; INORGANIC SULFATE; FUNCTIONAL-CHARACTERIZATION; BASOLATERAL MEMBRANE; EXPRESSION CLONING; KIDNEY CORTEX; REABSORPTION; SODIUM; AGE; COTRANSPORT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
54
Recensione:
Indirizzi per estratti:
Indirizzo: Morris, ME SUNY Buffalo, Sch Pharm, Dept Pharmaceut, 527 Hochstetter Hall,Buffalo, NY 14260 USA SUNY Buffalo 527 Hochstetter Hall Buffalo NY USA 14260 4260 USA
Citazione:
H.J. Lee et al., "Effect of pregnancy, postnatal growth, and gender on renal sulfate transport", P SOC EXP M, 221(4), 1999, pp. 336-344

Abstract

Serum sulfate concentrations are increased in infants, young children, andpregnant women, compared with adult values. The objective of this investigation was to examine the influences of age, gender, and pregnancy on renal sulfate transport using guinea pigs as an animal model, Membrane vesicles were isolated from the kidney cortex of male animals at four different ages,from male and female adult animals, and from pregnant and nonpregnant female animals. There were no significant differences in marker enzymes for thebrush-border membrane (BBM) or basolateral membrane (BLM) among all groupsexamined, Uptake was determined by a rapid filtration method and membrane fluidity by measuring the steady-state fluorescence anisotropy of 1,6-diphenyl-1,3,5-hexatriene. The V-max values for Na+/sulfate cotransport in BBM were significantly increased with decreasing age, whereas the K-m for this process was unchanged. The V-max and K-m for Na+/sulfate co-transport in BBMof pregnant animals were significantly higher than the values in the nonpregnant group. Bicarbonate-driven anion exchange of sulfate in BLM was not different among the different age groups. The V-max for the bicarbonate/sulfate exchange process in BLM was not different between pregnant and nonpregnant groups; however, the K-m for this process in BLM of pregnant animals was significantly greater than the value in nonpregnant animals. There were no gender-related differences in sulfate transport in BBM or BLM isolated from adult male and female animals. Renal BBM fluidity was increased with decreasing age and in pregnant animals, suggesting that altered membrane fluidity may represent one possible mechanism to explain the increased sodium/sulfate uptake in young and pregnant animals. The higher V-max for Na+/sulfate co-transport in young and pregnant animals suggests that there is an increased density of co-transporter protein or an increase in the rate of movement of the carrier protein (i.e., turnover) once loaded with sodium and sulfate. This increased conservation of inorganic sulfate in young and pregnant guinea pigs may be related to the increased demand for sulfated substrates, such as sulfated glycosaminoglycans, during growth and development.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 22:25:37