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Titolo:
STARCH SYNTHESIS IN AMYLOPLASTS PURIFIED FROM DEVELOPING POTATO-TUBERS
Autore:
NAEEM M; TETLOW IJ; EMES MJ;
Indirizzi:
UNIV MANCHESTER,SCH BIOL SCI,3614 STOPFORD BLDG MANCHESTER M13 9PT LANCS ENGLAND UNIV MANCHESTER,SCH BIOL SCI MANCHESTER M13 9PT LANCS ENGLAND
Titolo Testata:
Plant journal
fascicolo: 5, volume: 11, anno: 1997,
pagine: 1095 - 1103
SICI:
0960-7412(1997)11:5<1095:SSIAPF>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEXOSE-PHOSPHATE TRANSLOCATOR; ADP-GLUCOSE PYROPHOSPHORYLASE; CAULIFLOWER-BUD PLASTIDS; INORGANIC-PHOSPHATE; WHEAT ENDOSPERM; CARBOHYDRATE OXIDATION; SUSPENSION-CULTURES; ENVELOPE MEMBRANES; SUCROSE SYNTHASE; TRANSPORT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
M. Naeem et al., "STARCH SYNTHESIS IN AMYLOPLASTS PURIFIED FROM DEVELOPING POTATO-TUBERS", Plant journal, 11(5), 1997, pp. 1095-1103

Abstract

Amyloplasts have been purified from potato tubers by mechanical homogenization and gravity sedimentation through Nycodenz. Based on the recovery and latency of organelle-specific marker enzymes, the recovery of amyloplasts is approximately 13%, exhibiting 65% intactness, with less than 1% contamination by other subcellular fractions. These preparations were able to synthesize starch from glucose-1-phosphate plus ATP, or ADP-glucose but not from glucose-6-phosphate. Rates of starch synthesis from glucose-l-phosphate plus ATP were linear for up to 1 h andsensitive to the inhibitor 4,4-diisothiocyanato-stilbene 2,2-disulphonic acid (DIDS). Starch synthesis was optimal at pH 7.0 and was saturated by 5-10 mM glucose-1-phosphate and by 1 mM ADP-glucose. The results are discussed in the context of the pathway of starch synthesis and the transport of metabolites across the amyloplast envelope.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 12:23:25