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Titolo:
Effective induction of simian immunodeficiency virus-specific cytotoxic T lymphocytes in macaques by using a multiepitope gene and DNA prime-modifiedvaccinia virus Ankara boost vaccination regimen
Autore:
Hanke, T; Samuel, RV; Blanchard, TJ; Neumann, VC; Allen, TM; Boyson, JE; Sharpe, SA; Cook, N; Smith, GL; Watkins, DI; Cranage, MP; McMichael, AJ;
Indirizzi:
Univ Oxford, Inst Mol Med, Oxford OX3 9DS, England Univ Oxford Oxford England OX3 9DS Inst Mol Med, Oxford OX3 9DS, England Univ Oxford, Sir William Dunn Sch Pathol, Oxford OX1 3RE, England Univ Oxford Oxford England OX1 3RE n Sch Pathol, Oxford OX1 3RE, England Publ Hlth Lab Serv, Ctr Appl Microbiol & Res, Salisbury SP4 0JG, Wilts, England Publ Hlth Lab Serv Salisbury Wilts England SP4 0JG P4 0JG, Wilts, England PowderJect Vaccines Inc, Madison, WI 53711 USA PowderJect Vaccines Inc Madison WI USA 53711 s Inc, Madison, WI 53711 USA Univ Wisconsin, Wisconsin Reg Primate Res Ctr, Madison, WI 53715 USA Univ Wisconsin Madison WI USA 53715 rimate Res Ctr, Madison, WI 53715 USA
Titolo Testata:
JOURNAL OF VIROLOGY
fascicolo: 9, volume: 73, anno: 1999,
pagine: 7524 - 7532
SICI:
0022-538X(199909)73:9<7524:EIOSIV>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
INFECTED RHESUS-MONKEYS; I PEPTIDE COMPLEX; CELL RESPONSES; CTL EPITOPE; IMMUNE-RESPONSES; PROTECTIVE EFFICACY; DENDRITIC CELLS; HIV-1 INFECTION; TYPE-1; ANTIGEN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
74
Recensione:
Indirizzi per estratti:
Indirizzo: Hanke, T Univ Oxford, John Radcliffe Hosp, Inst Mol Med, Oxford OX3 9DS, England Univ Oxford Oxford England OX3 9DS Med, Oxford OX3 9DS, England
Citazione:
T. Hanke et al., "Effective induction of simian immunodeficiency virus-specific cytotoxic T lymphocytes in macaques by using a multiepitope gene and DNA prime-modifiedvaccinia virus Ankara boost vaccination regimen", J VIROLOGY, 73(9), 1999, pp. 7524-7532

Abstract

DNA and modified vaccinia virus Ankara (MVA) are vaccine vehicles suitableand safe for use in humans. Here, by using a multicytotoxic T-lymphocyte (CTL) epitope gene and a DNA prime-MVA boost vaccination regimen, high levels of CTLs specific for a single simian immunodeficiency virus (SN) gag-derived epitope were elicited in rhesus macaques. These vaccine-induced CTLs were capable of killing SIV-infected cells in vitro. Fluorescence-activated cell sorter analysis using soluble tetrameric major histocompatibility complex-peptide complexes showed that the vaccinated animals had 1 to 5% circulating CD8(+) lymphocytes specific for the vaccine epitope, frequencies comparable to those in SIV-infected monkeys. Upon intrarectal challenge with pathogenic SIVmac251, no evidence for protection was observed in at least two of the three vaccinated animals. This study does not attempt to define correlates of protective immunity nor design a protective vaccine against immunodeficiency viruses, but it demonstrates clearly that the DNA prime-MVA boost regimen is an effective protocol for induction of CTLs in macaques. It also shows that powerful tools for studying the role of CTLs in the control of SN and human immunodeficiency virus infections are now available: epitope-based vaccines, a protocol for an effective induction of CTLs in primates, and a simple and sensitive method for quantitation of epitope-specific T cells. The advantages of the DNA prime-MVA boost regimen as well as the correlations of tetramer staining of peripheral blood lymphocytes with CTL killing in vitro and postchallenge control of viremia are discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 14:50:51