Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Identification of genetic variation among St. Louis encephalitis virus isolates, using single-strand conformation polymorphism analysis
Autore:
Chandler, LJ; Nordoff, NG;
Indirizzi:
Univ Texas, Med Branch, Dept Pathol, Galveston, TX 77555 USA Univ Texas Galveston TX USA 77555 h, Dept Pathol, Galveston, TX 77555 USA Univ Texas, Med Branch, Ctr Trop Dis, Galveston, TX 77555 USA Univ Texas Galveston TX USA 77555 , Ctr Trop Dis, Galveston, TX 77555 USA
Titolo Testata:
JOURNAL OF VIROLOGICAL METHODS
fascicolo: 2, volume: 80, anno: 1999,
pagine: 169 - 178
SICI:
0166-0934(199907)80:2<169:IOGVAS>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEPATITIS-C; STRAINS; DNA; RNA; MUTATIONS;
Keywords:
arbovirus; flavivirus; RT-PCR; single-strand conformation polymorphism;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Chandler, LJ Univ Texas, Med Branch, Dept Pathol, Galveston, TX 77555 USA Univ Texas Galveston TX USA 77555 l, Galveston, TX 77555 USA
Citazione:
L.J. Chandler e N.G. Nordoff, "Identification of genetic variation among St. Louis encephalitis virus isolates, using single-strand conformation polymorphism analysis", J VIROL MET, 80(2), 1999, pp. 169-178

Abstract

A single-strand conformation polymorphism (SSCP) technique was developed for identification of genetic variation among 26 isolates of St. Louis encephalitis (SLE) virus. A 750-bp portion of the envelope gene was amplified byreverse transcription-polymerase chain reaction (RT-PCR) and the products analyzed by SSCP. SSCP reliably identified genetic variation among the isolates from the US, Central and South America. Closely related isolates from a smaller geographic area (Panama) were also distinguishable by SSCP. The sensitivity of this technique was demonstrated by sequencing each of the isolates used; SSCP was capable of discriminating between isolates that had asfew as 1-6 nucleotide differences. These results indicate that SSCP has excellent potential as a tool to screen rapidly SLE virus isolates for genetic variation and could be incorporated into molecular epidemiology studies. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 17:49:23