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Titolo:
Chemotactic movement and attachment of Agrobacterium tumefaciens to bananacells and tissues
Autore:
Hernandez, JBP; Remy, S; Sauco, VG; Swennen, R; Sagi, L;
Indirizzi:
Catholic Univ Louvain, Lab Trop Crop Improvement, B-3001 Heverlee, BelgiumCatholic Univ Louvain Heverlee Belgium B-3001 , B-3001 Heverlee, Belgium
Titolo Testata:
JOURNAL OF PLANT PHYSIOLOGY
fascicolo: 2, volume: 155, anno: 1999,
pagine: 245 - 250
SICI:
0176-1617(199908)155:2<245:CMAAOA>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLANTS; BACTERIA; ROOTS; MUSA;
Keywords:
Agrobacterium tumefaciens; bacterial attachment; banana; chemotaxis; genetic transformation; Musa spp; tissue culture;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Sagi, L Inst Canario Invest Agr, Dept Trop Fruits, Apartado 60, La Laguna 38080, Spain Inst Canario Invest Agr Apartado 60 La Laguna Spain 38080 , Spain
Citazione:
J.B.P. Hernandez et al., "Chemotactic movement and attachment of Agrobacterium tumefaciens to bananacells and tissues", J PLANT PHY, 155(2), 1999, pp. 245-250

Abstract

For the elaboration of efficient Agrobacterium-mediated transformation of banana the initial steps in the plant-bacterium interaction were investigated. Chemotaxis of agrobacteria towards tissues was studied using a swarm agar plate system. Excised leaf, rhizome, root and in vitro proliferating tissues from different banana landraces were found to be able to elicit a positive chemotactic reaction of A. tumefaciens that could be enhanced by extensive wounding. Agrobacterium attachment was examined by UV fluorescence microscopy and scanning electron microscopy, which demonstrated the presence of bacteria individually bound or massively attached to the surface of single banana cells and tissues. It can be concluded that at least during the two early steps of interaction A. tumefaciens appears to be compatible with banana, indicating the potential for genetic transformation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 15:49:08