Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Choline uptake by mouse brain capillary endothelial cells in culture
Autore:
Sawada, N; Takanaga, H; Matsuo, H; Naito, M; Tsuruo, T; Sawada, Y;
Indirizzi:
Kyushu Univ, Fac Pharmaceut Sci, Higashi Ku, Fukuoka 8128582, Japan KyushuUniv Fukuoka Japan 8128582 ci, Higashi Ku, Fukuoka 8128582, Japan Univ Tokyo, Inst Mol & Cellular Biosci, Bunkyo Ku, Tokyo, Japan Univ Tokyo Tokyo Japan t Mol & Cellular Biosci, Bunkyo Ku, Tokyo, Japan
Titolo Testata:
JOURNAL OF PHARMACY AND PHARMACOLOGY
fascicolo: 7, volume: 51, anno: 1999,
pagine: 847 - 852
SICI:
0022-3573(199907)51:7<847:CUBMBC>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
ORGANIC CATION TRANSPORTER; XENOPUS-LAEVIS OOCYTES; FUNCTIONAL EXPRESSION; P-GLYCOPROTEIN; NEURONS; BARRIER; ROCT1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Sawada, Y Kyushu Univ, Fac Pharmaceut Sci, Higashi Ku, 3-1-1 Maidashi, Fukuoka 8128582, Japan Kyushu Univ 3-1-1 Maidashi Fukuoka Japan 8128582 8128582, Japan
Citazione:
N. Sawada et al., "Choline uptake by mouse brain capillary endothelial cells in culture", J PHARM PHA, 51(7), 1999, pp. 847-852

Abstract

Choline, a precursor of the neurotransmitter acetylcholine, is synthesizedin only small amounts in the brain, so the choline concentration in the brain may vary depending on the plasma concentration and the transport rate across the blood-brain barrier. To elucidate the transport mechanism of choline, we carried out uptake experiments with mouse brain capillary endothelial cells in culture (MBEC4).[H-3]Choline uptake was linear for up to 5 min. An examination of the concentration dependence of [H-3]choline uptake revealed the operation of both saturable (J(max) = 423 +/- (mg protein)(-1) and K-t = 20.0 +/- 3.1 mu M) and non-saturable (k(d) = 1.23 +/- 0.045 mu L min(-1) (mg protein)(-1)) processes. The saturable process was independent of Na+ and pH, but was dependent on membrane potential as a driving force. Various basic drugs and endogenous substances, including substrates and inhibitors of the organic cation transporter, significantly inhibited the [H-3]choline uptake. These data suggest that choline was taken up into the endothelial cells via two routes and that a membrane potential-dependent carrier-mediated transport system may participate in choline transport across the blood-brain barrier.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 06:33:49