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Titolo:
Palindromic sequences and A plus T-rich DNA elements promote illegitimate recombination in Nicotiana tabacum
Autore:
Muller, AE; Kamisugi, Y; Gruneberg, R; Niedenhof, I; Horold, RJ; Meyer, P;
Indirizzi:
Univ Leeds, Fac Biol Sci, Leeds Inst Plant Biotechnol & Agr, Leeds LS2 9JT, W Yorkshire, England Univ Leeds Leeds W Yorkshire England LS2 9JT S2 9JT, W Yorkshire, England Max Planck Gesell, Max Delbruck Lab, D-50829 Cologne, Germany Max Planck Gesell Cologne Germany D-50829 Lab, D-50829 Cologne, Germany
Titolo Testata:
JOURNAL OF MOLECULAR BIOLOGY
fascicolo: 1, volume: 291, anno: 1999,
pagine: 29 - 46
SICI:
0022-2836(19990806)291:1<29:PSAAPT>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
DOUBLE-STRAND BREAKS; TRANSFORMATION BOOSTER SEQUENCE; GLOBIN GENE-CLUSTER; TOPOISOMERASE-II; ESCHERICHIA-COLI; INVERTED REPEATS; HOMOLOGOUS RECOMBINATION; SACCHAROMYCES-CEREVISIAE; SECONDARY STRUCTURE; DELETION FORMATION;
Keywords:
illegitimate recombination; DNA cruciform structure; SAR element; copy-choice model; breakage-and-rejoining model;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
91
Recensione:
Indirizzi per estratti:
Indirizzo: Meyer, P Univ Leeds, Fac Biol Sci, Leeds Inst Plant Biotechnol & Agr, Leeds LS2 9JT, W Yorkshire, England Univ Leeds Leeds W Yorkshire England LS2 9JT Yorkshire, England
Citazione:
A.E. Muller et al., "Palindromic sequences and A plus T-rich DNA elements promote illegitimate recombination in Nicotiana tabacum", J MOL BIOL, 291(1), 1999, pp. 29-46

Abstract

Illegitimate recombination is the prevailing molecular mechanism for the integration of recombinant DNA into the genome of most eukaryotic systems and the generation of deletions by intrachromosomal recombination. We developed a ?selectable marker system to screen for intrachromosomal illegitimate recombination events in order to assess the sequence and structure-specificrequirements for illegitimate recombination in tobacco. In 12 illegitimaterecombination products analysed, we found that all deletion termini localise to sites of palindromic structures or to A+T-rich DNA elements. All deletion termini showed microhomologies of two to six nucleotides. In three plants, the recombination products contained filler-DNA or an inversion of an endogenous segment. Our data strongly suggest that illegitimate recombination in plants is mediated by a DNA synthesis-dependent process, and that this mechanism is promoted by DNA regions that can form palindromic structuresor facilitate DNA unwinding. (C) 1999 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 14:06:48