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Titolo:
Epstein-Barr virus lacking latent membrane protein 2 immortalizes B cells with efficiency indistinguishable from that of wild-type virus
Autore:
Speck, P; Kline, KA; Cheresh, P; Longnecker, R;
Indirizzi:
Northwestern Univ, Sch Med, Dept Microbiol Immunol, Chicago, IL 60611 USA Northwestern Univ Chicago IL USA 60611 iol Immunol, Chicago, IL 60611 USA
Titolo Testata:
JOURNAL OF GENERAL VIROLOGY
, volume: 80, anno: 1999,
parte:, 8
pagine: 2193 - 2203
SICI:
0022-1317(199908)80:<2193:EVLLMP>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
GREEN FLUORESCENT PROTEIN; GROWTH TRANSFORMATION INVITRO; BLOOD MONONUCLEAR-CELLS; LYMPHOCYTE INFECTION; SIGNAL-TRANSDUCTION; MARMOSET LEUKOCYTES; TYROSINE KINASES; GENE-EXPRESSION; LMP2A; MUTANTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Longnecker, R Northwestern Univ, Sch Med, Dept Microbiol Immunol, Room 6-231,Ward Bldg,303 E Chicago Ave, Chicago, IL 60611 USA Northwestern Univ Room 6-231,Ward Bldg,303 E Chicago Ave Chicago IL USA 60611
Citazione:
P. Speck et al., "Epstein-Barr virus lacking latent membrane protein 2 immortalizes B cells with efficiency indistinguishable from that of wild-type virus", J GEN VIROL, 80, 1999, pp. 2193-2203

Abstract

Epstein-Barr virus (EBV) is a human herpesvirus that efficiently transforms and immortalizes human primary B lymphocytes, In this study, the role of latent membrane protein 2 (LMP2) in EBV growth transformation was investigated. LMP2 is a virally encoded membrane protein expressed in EBV-immortalized B cells previously shown to be nonessential for EBV transformation. However, a recent study reported that LMP2 may be an important determinant for efficient B cell transformation (Brielmeier et al., Journal of General Virology 77, 2807-2818, 1996), In this study a deletion mutation was introducedinto the LMP2 gene using an E. coil mini-EBV construct containing sufficient EBV DNA to result in growth transformation of primary B cells. In an alternative approach, the introduction of the gene encoding the enhanced greenfluorescent protein (EGFP) by homologous recombination into the LMP2 gene of EBV strain B95-8, generating the same LMP2 deletion mutation is reported. Careful quantification of B cell transformation using the EGFP(+)LMP2(-) recombinant virus determined that in liquid culture medium or in culture medium containing soft agarose there was no difference in the ability of LMP2(-) virus to immortalize primary human B cells when compared to that of wild-type virus.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/07/20 alle ore 07:50:14