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Titolo:
Type 3 and type 1 ryanodine receptors are localized in triads of the same mammalian skeletal muscle fibers
Autore:
Flucher, BE; Conti, A; Takeshima, H; Sorrentino, V;
Indirizzi:
Innsbruck Univ, Dept Physiol, A-6020 Innsbruck, Austria Innsbruck Univ Innsbruck Austria A-6020 ysiol, A-6020 Innsbruck, Austria San Raffaele Sci Inst, DIBIT, I-20132 Milan, Italy San Raffaele Sci Inst Milan Italy I-20132 t, DIBIT, I-20132 Milan, Italy Univ Tokyo, Dept Pharmacol, Fac Med, Tokyo 113, Japan Univ Tokyo Tokyo Japan 113 yo, Dept Pharmacol, Fac Med, Tokyo 113, Japan Univ Siena, Dept Biomed Sci, I-53100 Siena, Italy Univ Siena Siena ItalyI-53100 na, Dept Biomed Sci, I-53100 Siena, Italy
Titolo Testata:
JOURNAL OF CELL BIOLOGY
fascicolo: 3, volume: 146, anno: 1999,
pagine: 621 - 629
SICI:
0021-9525(19990809)146:3<621:T3AT1R>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
CALCIUM-RELEASE CHANNEL; SARCOPLASMIC-RETICULUM; MICE LACKING; CA2+ RELEASE; MOLECULAR-CLONING; CARDIAC-MUSCLE; MONOCLONAL-ANTIBODY; PROTEIN ISOFORMS; CONTRACTION; CELLS;
Keywords:
skeletal muscle; calcium release channel; excitation-contraction coupling immunofluorescence; knockout mice;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Flucher, BE Innsbruck Univ, Dept Physiol, Fritz Pregl Str 3, A-6020 Innsbruck, Austria Innsbruck Univ Fritz Pregl Str 3 Innsbruck Austria A-6020 ria
Citazione:
B.E. Flucher et al., "Type 3 and type 1 ryanodine receptors are localized in triads of the same mammalian skeletal muscle fibers", J CELL BIOL, 146(3), 1999, pp. 621-629

Abstract

The type 3 ryanodine receptor (RyR3) is a ubiquitous calcium release channel that has recently been found in mammalian skeletal muscles. However, in contrast to the skeletal muscle isoform (RyR1), neither the subcellular distribution nor the physiological role of RyR3 are known. Here, we used isoform-specific antibodies to localize RyR3 in muscles of normal and RyR knockout mice. In normal hind limb and diaphragm muscles of young mice, RyR3 was expressed in all fibers where it was codistributed with RyR1 and with the skeletal muscle dihydropyridine receptor. This distribution pattern indicates that RyR3 is localized in the triadic junctions between the transverse tubules and the sarcoplasmic reticulum. During development, RyR3 expression declined rapidly in some fibers whereas other fibers maintained expression of RyR3 into adulthood. Comparing the distribution of RyR3-containing fiberswith that of known fiber types did not show a direct correlation. Targeteddeletion of the RyR1 or RyR3 gene resulted in the expected loss of the targeted isoform, but had no adverse effects on the expression and localization of the respective other RyR isoform, The localization of RyR3 in skeletalmuscle triads, together with RyR1, is consistent with an accessory function of RyR3 in skeletal muscle excitation-contraction coupling.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 15:22:33