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Titolo:
Potentiation of endothelial cell proliferation by fibrin(ogen)-bound fibroblast growth factor-2
Autore:
Sahni, A; Sporn, LA; Francis, CW;
Indirizzi:
Univ Rochester, Med Ctr, Dept Med, Vasc Med Unit,Sch Med & Dent, Rochester, NY 14642 USA Univ Rochester Rochester NY USA 14642 Med & Dent, Rochester, NY 14642 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 21, volume: 274, anno: 1999,
pagine: 14936 - 14941
SICI:
0021-9258(19990521)274:21<14936:POECPB>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMINOGEN-ACTIVATOR PRODUCTION; DNA-SYNTHESIS; BETA-CHAIN; EXTRACELLULAR-MATRIX; VONWILLEBRAND-FACTOR; AFFINITY RECEPTORS; FIBRIN; EXPRESSION; HEPARIN; PROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Francis, CW Univ Rochester, Med Ctr, Dept Med, Vasc Med Unit,Sch Med & Dent, Box 610,601 Elmwood Ave, Rochester, NY 14642 USA Univ Rochester Box 610,601 Elmwood Ave Rochester NY USA 14642
Citazione:
A. Sahni et al., "Potentiation of endothelial cell proliferation by fibrin(ogen)-bound fibroblast growth factor-2", J BIOL CHEM, 274(21), 1999, pp. 14936-14941

Abstract

Endothelial cell growth is stimulated by fibroblast growth factor-2 (FGF-2), and both adhesion and proliferation are modulated by interactions with fibrinogen and fibrin. Previous evidence indicates that FGF-2 binds specifically and with high affinity to fibrinogen and fibrin, suggesting that theireffects on endothelial cells may be coordinated. In this study, we have, therefore, investigated the ability of FGF-2 bound to fibrinogen and fibrin to stimulate proliferation of endothelial cells. Human umbilical vein endothelial cells were cultured in the presence of FGF-2 with or without fibrinogen, and proliferation was assessed by microscopic examination of cultures,incorporation of [H-3]thymidine and by cell counting. Cells cultured in the presence of both FGF-2 and fibrinogen proliferated more rapidly than those with FGF-2 alone and exhibited a decreased population doubling time. At concentrations of FGF-2 up to 150 ng/ml, there was greater endothelial cell proliferation in the presence of fibrinogen than in its absence with the most pronounced effect below 1 ng/ml. The maximum effect of fibrinogen was observed at a molar ratio of fibrinogen to FGF-2 of 2:1, corresponding to themaximum molar binding ratio. Endothelial cells proliferated when plated onfibrin or surface-immobilized fibrinogen with FGF-2, indicating that FGF-2bound to surface-associated fibrin(ogen) retained activity. We conclude that fibrinogen- or fibrin-bound FGF-2 is able to support endothelial cell proliferation and that fibrinogen potentiates the proliferative capacity of FGF-2.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 16:03:56