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Titolo: The effect of pH and culture system on the attachment of plasmid-bearing and plasmid-cured Yersinia enterocolitica to a polycarbonate membrane in a surface adhesion immunofluorescent technique
Autore: Duffy, G; Sheridan, JJ;
- Indirizzi:
- TEAGASC, Natl Food Ctr, Dublin 15, Ireland TEAGASC Dublin Ireland 15TEAGASC, Natl Food Ctr, Dublin 15, Ireland
- Titolo Testata:
- JOURNAL OF APPLIED MICROBIOLOGY
fascicolo: 5,
volume: 86,
anno: 1999,
pagine: 867 - 873
- SICI:
- 1364-5072(199905)86:5<867:TEOPAC>2.0.ZU;2-6
- Fonte:
- ISI
- Lingua:
- ENG
- Soggetto:
- LISTERIA-MONOCYTOGENES; BACTERIAL ATTACHMENT; RAPID DETECTION; MEAT; TEMPERATURE; POLYSTYRENE; ADSORPTION; RECOVERY; PRODUCTS; INNOCUA;
- Tipo documento:
- Article
- Natura:
- Periodico
- Settore Disciplinare:
- Agriculture,Biology & Environmental Sciences
- Life Sciences
- Citazioni:
- 22
- Recensione:
- Indirizzi per estratti:
- Indirizzo: Duffy, G TEAGASC, Natl Food Ctr, Dublin 15, Ireland TEAGASC Dublin Ireland 15 SC, Natl Food Ctr, Dublin 15, Ireland
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- Citazione:
- G. Duffy e J.J. Sheridan, "The effect of pH and culture system on the attachment of plasmid-bearing and plasmid-cured Yersinia enterocolitica to a polycarbonate membrane in a surface adhesion immunofluorescent technique", J APPL MICR, 86(5), 1999, pp. 867-873
Abstract
A rapid surface adhesion based immunofluorescence technique was used to isolate and detect Yersinia enterocolitica from inoculated enriched culture systems. The pathogen was isolated by surface adhesion to a polycarbonate membrane which was mounted on a glass slide and immersed in the enriched culture for 15 min. The pathogen was detected using a fluorescent labelled (FITC) monoclonal antibody which was specific for Y. enterocolitica serotype O:3 and then viewed using fluorescent microscopy. The effect of culture type (broth, meat homogenate and minced meat) and pH (5.00, 7.00, 9.00 and 11.00) on the adhesion of plasmid-bearing and plasmid-cured Y. enterocolitica tothe polycarbonate membrane in this technique was determined. The pH had a significant effect (P < 0.05) in broth and meat homogenate cultures, with enhanced attachment of Y. enterocolitica (P+ and P-) at pH 9.00 than at pH 5, 7 or 11. Culture type was also important, with differences observed in the numbers of Yersinia adhering to membranes immersed in broth, meat homogenate and minced beef: Differences in attachment were noted between plasmid-bearing and plasmid-cured Y. enterocolitica isolated from similar cultural environments. The reasons for these observed differences, and their implications for the surface adhesion immunofluorescent rapid method, are discussed.
ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 15:53:19