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Titolo:
Inhibition of HIV-1 replication by a two-strand system (FTFOs) targeted tothe polypurine tract
Autore:
Hiratou, T; Tsukahara, S; Miyano-Kurosaki, N; Takai, K; Yamamoto, N; Takaku, H;
Indirizzi:
Chiba Inst Technol, Dept Ind Chem, Narashino, Chiba 2750016, Japan Chiba Inst Technol Narashino Chiba Japan 2750016 no, Chiba 2750016, Japan Tokyo Med & Dent Univ, Sch Med, Dept Microbiol, Bunkyo Ku, Tokyo 1138519, Japan Tokyo Med & Dent Univ Tokyo Japan 1138519 unkyo Ku, Tokyo 1138519, Japan
Titolo Testata:
FEBS LETTERS
fascicolo: 1, volume: 456, anno: 1999,
pagine: 186 - 190
SICI:
0014-5793(19990730)456:1<186:IOHRBA>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRIPLE-HELIX FORMATION; HUMAN-IMMUNODEFICIENCY-VIRUS; DEPENDENT DNA POLYMERASE; FORMING OLIGONUCLEOTIDES; VIRAL-RNA; PHOSPHOROTHIOATE OLIGODEOXYNUCLEOTIDE; REVERSE TRANSCRIPTION; BINDING; INVITRO; SEQUENCES;
Keywords:
triplex; two-strand system; inhibition of human immunodeficiency virus replication; polypurine tract; MOLT-4 cell; MT-4 cell;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Takaku, H Chiba Inst Technol, Dept Ind Chem, Narashino, Chiba 2750016, Japan Chiba Inst Technol Narashino Chiba Japan 2750016 2750016, Japan
Citazione:
T. Hiratou et al., "Inhibition of HIV-1 replication by a two-strand system (FTFOs) targeted tothe polypurine tract", FEBS LETTER, 456(1), 1999, pp. 186-190

Abstract

Reverse transcription of HIV-1 vRNA into, the double-stranded DNA provirusinvolves initiation of pins-strand DNA synthesis at the polypurine tract (PPT) by reverse transcriptase (RT), The PPT is highly conserved among the known human immunodeficiency virus (HIV-1) strains and is a possible target for triplex formation, We show the effects of triple-helix formation by assays of primer extension inhibition in vitro, using a two-strand system (foldback triplex-forming oligonucleotides (FTFOs)) targeted to the PPT of HIV-1, The two-stranded composition of a triple-helix is thermodynamically and kinetically superior to the three-strand system. The FTFOs inhibited the RTactivity in a sequence-specific manner, i.e. the tripler actually formed at the PPT and blocked the RT, The FTFOs containing the phosphorothioate groups at the antisense sequences showed greater 3'-exonuclease resistance. InHIV-1-infected MOLT-4 cells, the FTFOs containing the phosphorothioate groups at the antisense sequence sites and guanosine rich parts within the third Hoogsteen base-pairing sequence inhibit the replication of HIV-1 more effectively than the antisense oligonucleotides, indicating sequence-specificinhibition of HIV-1 replication. (C) 1999 Federation of European Biochemical Societies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 20:45:33