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Titolo:
Accommodation of foreign genes into the Sendai virus genome: sizes of inserted genes and viral replication
Autore:
Sakai, Y; Kiyotani, K; Fukumura, M; Asakawa, M; Kato, A; Shioda, T; Yoshida, T; Tanaka, A; Hasegawa, M; Nagai, Y;
Indirizzi:
Univ Tokyo, Inst Med Sci, Div Viral Infect, Minato Ku, Tokyo, Japan Univ Tokyo Tokyo Japan d Sci, Div Viral Infect, Minato Ku, Tokyo, Japan Univ Tokyo, Inst Med Sci, Dept Infect Dis, Tokyo, Japan Univ Tokyo TokyoJapan kyo, Inst Med Sci, Dept Infect Dis, Tokyo, Japan Hiroshima Univ, Sch Med, Dept Bacteriol, Hiroshima, Japan Hiroshima Univ Hiroshima Japan ch Med, Dept Bacteriol, Hiroshima, Japan DNAVEC Res, Tsukuba, Ibaraki, Japan DNAVEC Res Tsukuba Ibaraki JapanDNAVEC Res, Tsukuba, Ibaraki, Japan Osaka City Univ, Sch Med, Dept Pediat, Osaka 545, Japan Osaka City Univ Osaka Japan 545 , Sch Med, Dept Pediat, Osaka 545, Japan
Titolo Testata:
FEBS LETTERS
fascicolo: 2, volume: 456, anno: 1999,
pagine: 221 - 226
SICI:
0014-5793(19990806)456:2<221:AOFGIT>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
V-PROTEIN; PATHOGENESIS; SDF-1-ALPHA; EXPRESSION; SDF-1-BETA; RNA;
Keywords:
Sendai virus; genome; replication;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
14
Recensione:
Indirizzi per estratti:
Indirizzo: Nagai, Y Univ Tokyo, Inst Med Sci, Div Viral Infect, Minato Ku, 4-6-1 Shirokanedai,Tokyo, Japan Univ Tokyo 4-6-1 Shirokanedai Tokyo Japan okanedai,Tokyo, Japan
Citazione:
Y. Sakai et al., "Accommodation of foreign genes into the Sendai virus genome: sizes of inserted genes and viral replication", FEBS LETTER, 456(2), 1999, pp. 221-226

Abstract

Sendai virus (SeV) is an enveloped virus with a negative sense genome RNA of about 15.3 kb, We previously established a system to recover an infectious virus entirely from SeV cDNA and illustrated the feasibility of using SeV as a novel expression vector, Here, me have attempted to insert a series of foreign genes into SeV of different lengths to learn how far SeV can accommodate extra genes and how the length of inserted genes affects viral replication in cells cultured in vitro and in the natural host, mice, We show that a gene up to 3.2 kb can be inserted and efficiently expressed and thatthe replication speed as,well as the final virus titers in cell culture are proportionally reduced as the inserted gene length increases, In vivo, such a size-dependent effect was not very clear but a remarkably attenuated replication and pathogenicity were generally seen. Our data further confirmed reinforcement of foreign gene expression in vitro from the V(-) version of SeV in which the accessory V gene had been knocked out. Based on these results, we discuss the utility of SeV vector in terms of both efficiency andsafety. (C) 1999 Federation of European Biochemical Societies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/20 alle ore 01:52:45