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Titolo:
Effect of nodularin on the expression of glutathione S-transferase placental form and proliferating cell nuclear antigen in N-nitrosodiethylamine initiated hepatocarcinogenesis in the male Fischer 344 rat
Autore:
Song, KY; Lim, IK; Park, SC; Lee, SO; Park, HS; Choi, YK; Hyun, BH;
Indirizzi:
Ajou Univ, Sch Med, Dept Biochem, Suwon 442749, South Korea Ajou Univ Suwon South Korea 442749 pt Biochem, Suwon 442749, South Korea Chung Ang Univ, Coll Med, Dept Pathol, Seoul 156756, South Korea Chung AngUniv Seoul South Korea 156756 athol, Seoul 156756, South Korea Seoul Natl Univ, Coll Med, Dept Biochem, Seoul, South Korea Seoul Natl Univ Seoul South Korea Med, Dept Biochem, Seoul, South Korea Korea Res Inst Biosci & Biotechnol, Yusong, South Korea Korea Res Inst Biosci & Biotechnol Yusong South Korea song, South Korea
Titolo Testata:
CARCINOGENESIS
fascicolo: 8, volume: 20, anno: 1999,
pagine: 1541 - 1548
SICI:
0143-3334(199908)20:8<1541:EONOTE>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEPATITIS-B VIRUS; HEPATOCELLULAR-CARCINOMA; C VIRUS; PROTEIN PHOSPHATASES; REPLICATION INVITRO; POTENT INHIBITOR; STRAND SYNTHESIS; TUMOR PROMOTION; ENDEMIC AREA; ALPHA GENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Lim, IK Ajou Univ, Sch Med, Dept Biochem, Suwon 442749, South Korea Ajou Univ Suwon South Korea 442749 em, Suwon 442749, South Korea
Citazione:
K.Y. Song et al., "Effect of nodularin on the expression of glutathione S-transferase placental form and proliferating cell nuclear antigen in N-nitrosodiethylamine initiated hepatocarcinogenesis in the male Fischer 344 rat", CARCINOGENE, 20(8), 1999, pp. 1541-1548

Abstract

The tumor-promoting effect of nodularin during carcinogenesis was investigated, Male Fischer 344 rats were injected with nodularin for 10 weeks from week 3 after N-nitrosodiethylamine initiation without partial hepatectomy. Rats were further maintained for 10 weeks after the cessation of nodularin and were periodically killed, In contrast to the minimal foci in the DEN and nodularin alone groups, treatment with DEN and nodularin produced four kinds of nodules with eosinophilic, clear, mixed and basophilic cells, After the cessation of nodularin, the maximally increased number, but not the area, of glutathione S-transferase placental form-positive [GST-P(+)] nodules at week 12 decreased significantly and the appearance of two types of hyperplastic nodules was noted by GST-P immunostaining; homogeneously stained dense nodules (DN) and heterogeneously stained pale nodules (PN), which appeared only after the cessation of nodularin, DN were well circumscribed by enzyme-altered cells, as opposed to poorly in PN, Moreover, normal-appearing hepatocytes replaced the enzyme-altered cells in PN, In contrast to the higher PCNA index in GST-P(+) DN, the background level returned to that of thecontrol at week 15, PCNA indices in DN were significantly higher than in PN, which were still higher than the control, indicating that nodularin affected the PCNA index differentially in the altered and unaltered hepatocytes. However, nodularin without DEN initiation significantly increased the PCNA index through initial cell death and subsequent hepatocyte proliferation,These results suggest that: (i) nodularin has a promoting effect by inducing hepatocyte proliferation in both enzyme-altered hyperplastic nodules andthe surrounding parenchyma; (ii) proliferation is transient in background cells but not in enzyme-altered hepatocytes; (iii) GST-P(+) DN can be regarded as progressive and GST-P(+) PN as regressive, revealed by both immunohistochemistry and PCNA index.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 07:39:29