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Titolo:
Electrostatic encapsulation and growth of plant cell cultures in alginate
Autore:
Al-Hajry, HA; Al-Maskry, SA; Al-Kharousi, LM; El-Mardi, O; Shayya, WH; Goosen, MFA;
Indirizzi:
Sultan Qaboos Univ, Coll Agr, Dept Bioresource & Agr Engn, Muscat, Oman Sultan Qaboos Univ Muscat Oman ept Bioresource & Agr Engn, Muscat, Oman Sultan Qaboos Univ, Coll Agr, Dept Agron Hort Entomol & Plant Pathol, Muscat, Oman Sultan Qaboos Univ Muscat Oman ort Entomol & Plant Pathol, Muscat, Oman
Titolo Testata:
BIOTECHNOLOGY PROGRESS
fascicolo: 4, volume: 15, anno: 1999,
pagine: 768 - 774
SICI:
8756-7938(199907/08)15:4<768:EEAGOP>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
DROPLET GENERATION; SHOOT TIPS; PROPAGATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Goosen, MFA Sultan Qaboos Univ, Coll Engn, POB 33, Al Khod 123, Oman Sultan Qaboos Univ POB 33 Al Khod Oman 123 Al Khod 123, Oman
Citazione:
H.A. Al-Hajry et al., "Electrostatic encapsulation and growth of plant cell cultures in alginate", BIOTECH PR, 15(4), 1999, pp. 768-774

Abstract

The growth of callus tissue from African Violets, encapsulated in alginateusing electrostatics, was investigated as well as the mechanism of alginate droplet formation. Alginate microbeads as small as 500 (+/-50) microns indiameter could be produced by electrostatic extrusion directly from a plastic syringe (1900 micron extrusion orifice), in the absence of a needle. Video analysis of the mechanism of electrostatic alginate droplet formation from the syringe showed the development of a Taylor cone-like droplet which extended to form a thin strand that then broke up into droplets. Autoclaving of the alginate/medium solution significantly reduced its viscosity, giving smaller beads. Calculated microbead diameters agreed well with experimental values. Callus tissue from leaf explants was successfully immobilized and cultured using electrostatic extrusion. Tissue immobilized using 4% alginate in medium and cultured on agar grew best, producing a complete plantlet within four months. The long-term aim is to develop an effective method for large production of artificial seeds.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/04/20 alle ore 02:07:50