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Titolo:
Oscillatory behavior of a simple kinetic model for proteolysis during cellinvasion
Autore:
Berry, H; Larreta-Garde, V;
Indirizzi:
Univ Cergy Pontoise, ERRMECE, F-95302 Cergy Pontoise, France Univ Cergy Pontoise Cergy Pontoise France F-95302 Cergy Pontoise, France
Titolo Testata:
BIOPHYSICAL JOURNAL
fascicolo: 2, volume: 77, anno: 1999,
pagine: 655 - 665
SICI:
0006-3495(199908)77:2<655:OBOASK>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
MATRIX METALLOPROTEINASES; PROTEIN-KINASE; TYROSINE PHOSPHORYLATION; FIBRONECTIN FRAGMENTS; PLASMINOGEN-ACTIVATOR; SIGNAL-TRANSDUCTION; PLASMA FIBRONECTIN; FIBROBLASTIC CELLS; MIGRATION SPEED; GENE-EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
66
Recensione:
Indirizzi per estratti:
Indirizzo: Larreta-Garde, V Univ Cergy Pontoise, ERRMECE, 2 Ave Adolphe Chauvin,BP 222, F-95302 Cergy Pontoise, France Univ Cergy Pontoise 2 Ave Adolphe Chauvin,BP 222 Cergy Pontoise France F-95302
Citazione:
H. Berry e V. Larreta-Garde, "Oscillatory behavior of a simple kinetic model for proteolysis during cellinvasion", BIOPHYS J, 77(2), 1999, pp. 655-665

Abstract

Extracellular proteolysis during cell invasion is thought to be tightly organized, both temporally and spatially. This work presents a simple kineticmodel that describes the interactions between extracellular matrix (ECM) proteins, proteinases, proteolytic fragments, and integrins. Nonmonotonous behavior arises from enzyme de novo synthesis consecutive to integrin binding to fragments or entire proteins. The model has been simulated using realistic values for kinetic constants and protein concentrations, with fibronectin as the ECM protein. The simulations show damped oscillations of integrin-complex concentrations, indicating alternation of maximal adhesion periods with maximal mobility periods. Comparisons with experimental data from the literature confirm the similarity between this system behavior and cell invasion. The influences on the system of cryptic functions of ECM proteins,proteinase inhibitors, and soluble antiadhesive peptides were examined. The first critical parameter for oscillation is the discrepancy between integrin affinity for intact ECM proteins and the respective proteolytic fragments, thus emphasizing the importance of cryptic functions of ECM proteins incell invasion. Another critical parameter is the ratio between proteinase and the initial ECM protein concentration. These results suggest new insights into the organization of the ECM degradation during cell invasion.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 07:38:54