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Titolo:
Characterization of the high-affinity monocarboxylate transporter MCT2 in Xenopus laevis oocytes
Autore:
Broer, S; Broer, A; Schneider, HP; Stegen, C; Halestrap, AP; Deitmer, JW;
Indirizzi:
Univ Tubingen, Inst Physiol Chem, D-72076 Tubingen, Germany Univ TubingenTubingen Germany D-72076 l Chem, D-72076 Tubingen, Germany Univ Kaiserslautern, Abt Allgemeine Zool, FB Biol, D-67653 Kaiserslautern,Germany Univ Kaiserslautern Kaiserslautern Germany D-67653 aiserslautern,Germany Univ Bristol, Sch Med Sci, Dept Biochem, Bristol BS8 1TD, Avon, England Univ Bristol Bristol Avon England BS8 1TD Bristol BS8 1TD, Avon, England
Titolo Testata:
BIOCHEMICAL JOURNAL
, volume: 341, anno: 1999,
parte:, 3
pagine: 529 - 535
SICI:
0264-6021(19990801)341:<529:COTHMT>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTRACELLULAR PH INDICATOR; LACTATE TRANSPORTER; SKELETAL-MUSCLE; INHIBITOR SPECIFICITY; HUMAN-ERYTHROCYTES; GLIAL-CELLS; LACTIC-ACID; NEURONS; CARRIER; EXPRESSION;
Keywords:
brain energy metabolism; expression; ketone bodies; lactate transport; pH regulation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Broer, S Univ Tubingen, Inst Physiol, Gmelinstr 5, D-72076 Tubingen, Germany Univ Tubingen Gmelinstr 5 Tubingen Germany D-72076 ngen, Germany
Citazione:
S. Broer et al., "Characterization of the high-affinity monocarboxylate transporter MCT2 in Xenopus laevis oocytes", BIOCHEM J, 341, 1999, pp. 529-535

Abstract

Observations on lactate transport in brain cells and cardiac myocytes indicate the presence of a high-affinity monocarboxylate transporter. The rat monocarboxylate transporter isoform MCT2 was analysed by expression in Xenopus laevis oocytes and the results were compared with the known characteristics of lactate transport in heart and brain. Monocarboxylate transport via MCT2 was driven by the H+ gradient over the plasma membrane. Uptake of lactate strongly increased with decreasing pH, showing half-maximal stimulationat pH 7.2. A wide variety of monocarboxylates and ketone bodies, includinglactate, pyruvate, beta-hydroxybutyrate, acetoacetate, 2-oxoiso-valerate and 2-oxoisohexanoate, were substrates of MCT2. All substrates had a high affinity for MCT2, For lactate a K-m value of 0.74 +/- 0.07 mM was determinedat pH 7.0. For the other substrates, K-i values between 100 mu M and 1 mM were measured for inhibition of lactate transport, which is about one-tenthof the corresponding values for the ubiquitously expressed monocarboxylatetransporter isoform MCT1. Monocarboxylate transport via MCT2 could be inhibited by alpha-cyano-4-hydroxycinnamate, anion-channel inhibitors and flavonoids. It is suggested that cells which express MCT2 preferentially use lactate and ketone bodies as energy sources.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/01/20 alle ore 17:33:56