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Titolo:
Up-regulation of growth hormone receptor immunoreactivity in human melanoma
Autore:
Lincoln, DT; Sinowatz, F; Kolle, S; Takahashi, H; Parsons, P; Waters, M;
Indirizzi:
Kuwait Univ, Fac Allied Hlth Sci, Dept Med Sci, Sulaibikhat 90805, Kuwait Kuwait Univ Sulaibikhat Kuwait 90805 Med Sci, Sulaibikhat 90805, Kuwait Univ Munich, Dept Vet Anat, Munich, Germany Univ Munich Munich GermanyUniv Munich, Dept Vet Anat, Munich, Germany Sapporo Med Ctr, Sapporo, Hokkaido, Japan Sapporo Med Ctr Sapporo Hokkaido Japan Med Ctr, Sapporo, Hokkaido, Japan Queensland Inst Med Res, Brisbane, Qld 4006, Australia Queensland Inst MedRes Brisbane Qld Australia 4006 , Qld 4006, Australia Univ Queensland, Dept Physiol & Pharmacol, St Lucia, Qld, Australia Univ Queensland St Lucia Qld Australia armacol, St Lucia, Qld, Australia
Titolo Testata:
ANTICANCER RESEARCH
fascicolo: 3A, volume: 19, anno: 1999,
pagine: 1919 - 1931
SICI:
0250-7005(199905/06)19:3A<1919:UOGHRI>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
BREAST-CANCER-CELLS; SERUM BINDING-PROTEIN; FACTOR-I; GENE-EXPRESSION; MALIGNANT-MELANOMA; EGF RECEPTOR; RAT-LIVER; C-MYC; SOMATOMEDIN; INVITRO;
Keywords:
melanoma; growth hormone; growth hormone receptor; immunohistochemistry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
82
Recensione:
Indirizzi per estratti:
Indirizzo: Lincoln, DT Kuwait Univ, Fac Allied Hlth Sci, Dept Med Sci, POB 31470, Sulaibikhat 90805, Kuwait Kuwait Univ POB 31470 Sulaibikhat Kuwait 90805 90805, Kuwait
Citazione:
D.T. Lincoln et al., "Up-regulation of growth hormone receptor immunoreactivity in human melanoma", ANTICANC R, 19(3A), 1999, pp. 1919-1931

Abstract

Growth hormone (GH),exerts its regulatory functions in controlling metabolism, balanced growth and differentiated cell expression by acting on specific receptors, which trigger a phosphorylation cascade resulting in the modulation of numerous signalling pathways, and dictate gene expression. Immunohistochemical techniques were used to demonstrate the presence of growth hormone receptors in 126 formalin-fixed, paraffin-embedded melanocytic tumours comprising melanocytic naevi, superficial spreading melanoma, nodular melanoma, lentigo maligna melanoma and metastatic melanomas. The relative proportion of positive cells and intensity of staining was higher in neoplasticcells, compared to normal cutaneous cells. Of the 76 cases of common melanocytic naevi (CMN) studies, 46 were weakly reactive with MAb 263. Heterogeneity of immunoreactivity was found in primary melanoma lesions with a variable range of positive cells. Of 37 cases studied 34 were moderately to strongly positive. Immunoreactivity showed subcellular localization of the GH-receptor in cell membranes, was predominantly cytoplasmic, but strong nuclear immunoreaction was also apparent in many instances. The nuclear localization of immunoreactivity is the result of nuclear GH-receptor/binding protein, identically to the cytosolic and plasma growth hormone binding protein. Intense immuno-reactivity was also observed in the cellular Golgi area of established cell lines and cultured tissue-derived cells in exponential growth phase, indicating cells are capable of GH-receptor synthesis. In the primary lesions, dermal tumour cells tended to be more immunoreactive relativeto those seen in the dermal region. Metastatic lesions in various organs also expressed growth hormone receptors in secondary tumour cells and all ofthe metastatic cases were positive. The expression of GH-receptors in human melanoma cells means that these cells are directly responsive to GH action and that GH may stimulate local production of IGF-I, which then acts in an autocrine mechanism.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 09:39:25