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Titolo:
Characterization of beta-lactam-resistant Bacteroides fragilis isolates byuse of PCR fingerprinting
Autore:
Fang, H; Hedberg, M; Edlund, C; Jarstrand, C; Fodor, E; Nord, CE;
Indirizzi:
Huddinge Univ Hosp, Karolinska Inst, Dept Immunol Microbiol Pathol & Infect Dis, S-14186 Huddinge, Sweden Huddinge Univ Hosp Huddinge Sweden S-14186 Dis, S-14186 Huddinge, Sweden Albert Szent Gyorgyi Med Univ, Dept Clin Microbiol, Szeged, Hungary AlbertSzent Gyorgyi Med Univ Szeged Hungary Microbiol, Szeged, Hungary Univ Coll S Stockholm, Stockholm, Sweden Univ Coll S Stockholm StockholmSweden l S Stockholm, Stockholm, Sweden
Titolo Testata:
ANAEROBE
fascicolo: 1, volume: 5, anno: 1999,
pagine: 11 - 18
SICI:
1075-9964(199902)5:1<11:COBBFI>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
ARBITRARY PRIMERS; GENE; IDENTIFICATION; STRAINS;
Keywords:
Bacteroides fragilis; PCR fingerprinting; beta-lactam; resistance; metallo-beta-lactamase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Hedberg, M Huddinge Univ Hosp, Karolinska Inst, Dept Immunol Microbiol Pathol & Infect Dis, S-14186 Huddinge, Sweden Huddinge Univ Hosp Huddinge Sweden S-14186 6 Huddinge, Sweden
Citazione:
H. Fang et al., "Characterization of beta-lactam-resistant Bacteroides fragilis isolates byuse of PCR fingerprinting", ANAEROBE, 5(1), 1999, pp. 11-18

Abstract

PCR fingerprinting was used for characterization of 35 beta-lactam-resistant Bacteroides fragilis strains isolated in Sweden and Hungary. Ten B. fragilis strains showed unique PCR fingerprints by use of the M13 core primer. Their main product was a DNA fragment with a length of 2000-bp which was absent in the other 25 strains and the reference strain B. fragilis ATCC 25285. The 2000-bp fragment from four imipenem-resistant strains gave rise to positive reactions in a specific PCR for detection of ccrA. Printed by the T3B primer, five B. fragilis strains, including the imipenem-resistant strains showed unique PCR fingerprints. The investigated imipenem-resistant strains produced carbapenem-hydrolysing metallo-beta-lactamases. The study indicates that the unique PCR fingerprinting profiles shown in highly p-lactam resistant B. fragilis strains are correlated to antimicrobial resistance. The PCR fingerprinting technique is a useful tool for differentiation of Bacteroides fragilis strains with high-level beta-lactam resistance. (C) 1999 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 08:11:43