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Titolo:
Polarized vascular endothelial growth factor secretion by human retinal pigment epithelium and localization of vascular endothelial growth factor receptors on the inner choriocapillaris - Evidence for a trophic paracrine relation
Autore:
Blaauwgeers, HGT; Hotkamp, BW; Rutten, H; Witmer, AN; Koolwijk, P; Partanen, TA; Alitalo, K; Kroon, ME; Kijlstra, A; van Hinsbergh, VWM; Schlingemann, RO;
Indirizzi:
Univ Amsterdam, Acad Med Ctr, Dept Ophthalmol, NL-1100 DD Amsterdam, Netherlands Univ Amsterdam Amsterdam Netherlands NL-1100 DD D Amsterdam, Netherlands TNO, IVVO, Gaubius Lab, NL-2300 AK Leiden, Netherlands TNO Leiden Netherlands NL-2300 AK us Lab, NL-2300 AK Leiden, Netherlands Univ Helsinki, Haartman Inst, Dept Pathol, Helsinki, Finland Univ Helsinki Helsinki Finland man Inst, Dept Pathol, Helsinki, Finland Netherlands Ophthalm Res Inst, Dept Ophthalmoimmunol, NL-1100 AC Amsterdam, Netherlands Netherlands Ophthalm Res Inst Amsterdam Netherlands NL-1100AC therlands
Titolo Testata:
AMERICAN JOURNAL OF PATHOLOGY
fascicolo: 2, volume: 155, anno: 1999,
pagine: 421 - 428
SICI:
0002-9440(199908)155:2<421:PVEGFS>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERMEABILITY FACTOR; IN-VITRO; SUBRETINAL NEOVASCULARIZATION; INCREASED EXPRESSION; CELLS PRODUCE; VEGF; ANGIOGENESIS; PROTEINS; PDGF; AGE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Schlingemann, RO Univ Amsterdam, Acad Med Ctr, Dept Ophthalmol, POB 22660,NL-1100 DD Amsterdam, Netherlands Univ Amsterdam POB 22660 Amsterdam Netherlands NL-1100 DD
Citazione:
H.G.T. Blaauwgeers et al., "Polarized vascular endothelial growth factor secretion by human retinal pigment epithelium and localization of vascular endothelial growth factor receptors on the inner choriocapillaris - Evidence for a trophic paracrine relation", AM J PATH, 155(2), 1999, pp. 421-428

Abstract

The retinal pigment epithelium (RPE) maintains the choriocapillaris (CC) in the normal eye and is involved in the pathogenesis of choroidal neovascularization in age-related macular degeneration. Vascular endothelial growth factor-A (VEGF) is produced by differentiated human RPE cells in vitro and in vitro and may be involved in paracrine signaling between the RPE and theCC. We investigated whether there is a polarized secretion of VEGF by RPE cells in vitro. Also, the localization of VEGF receptors in the human retina was investigated. we observed that highly differentiated human RPE cells,cultured on transwell filters in. normoxic conditions, produced two- to sevenfold more VEGF toward their basolateral side as compared to the apical side. In hypoxic conditions, VEGF-A secretion increased to the basal side only, resulting in a three- to 10-fold higher basolateral secretion. By immunohistochemistry in 50 human eyes and in two cynomolgus monkey eyes, KDR (VEGFR-2) and flt-4 (VEGFR-3) were preferentially localized at the side of theCC endothelium facing the RPE cell layer, whereas flt-1 (VEGFR-1) was found on the inner CC and on other choroidal vessels. Our results indicate thatRPE secretes VEGF toward its basal side where its receptor KDR is located on the adjacent CC endothelium, suggesting a role of VEGF in a paracrine relation, possibly in cooperation with flt-4 and its ligand, This can explainthe known trophic function of the RPE in the maintenance of the CC and itsfenestrated permeable phenotype and points to a role for VEGF in normal eye functioning. Up-regulated basolateral VEGF secretion by RPE in hypoxia orloss of polarity of VEGF production may play a role in the pathogenesis ofchoroidal neovascularization.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 15:44:40