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Titolo:
Human polyomavirus JC latency and reactivation status in blood of HIV-1-positive immunocompromised patients with and without progressive multifocal leukoencephalopathy
Autore:
Andreoletti, L; Dubois, V; Lescieux, A; Dewilde, A; Bocket, L; Fleury, HJA; Wattre, P;
Indirizzi:
Ctr Hosp Reg & Univ Lille, Virol Lab, F-59037 Lille, France Ctr Hosp Reg &Univ Lille Lille France F-59037 ab, F-59037 Lille, France Univ Bordeaux 2, Virol Lab, F-33076 Bordeaux, France Univ Bordeaux 2 Bordeaux France F-33076 ol Lab, F-33076 Bordeaux, France
Titolo Testata:
AIDS
fascicolo: 12, volume: 13, anno: 1999,
pagine: 1469 - 1475
SICI:
0269-9370(19990820)13:12<1469:HPJLAR>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; VIRUS-INFECTION; PERIPHERAL-BLOOD; MESSENGER-RNAS; LYMPHOCYTES; CELLS; BRAIN; DNA; AIDS; DIAGNOSIS;
Keywords:
human polyomavirus JC; JCV; JCV mRNA; progressive multifocal leukoencephalopathy; peripheral blood mononuclear cells; everse transcriptase polymerase chain reaction; immunocompromised patients; HIV-1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Andreoletti, L Ctr Hosp Reg & Univ Lille, Virol Lab, Batiment IRFPPS, F-59037 Lille, France Ctr Hosp Reg & Univ Lille Batiment IRFPPS Lille France F-59037
Citazione:
L. Andreoletti et al., "Human polyomavirus JC latency and reactivation status in blood of HIV-1-positive immunocompromised patients with and without progressive multifocal leukoencephalopathy", AIDS, 13(12), 1999, pp. 1469-1475

Abstract

Background: Human polyomavirus JC (JCV) induces human progressive multifocal leukoencephalopathy (PML) in patients with AIDS. Peripheral blood mononuclear cells (PBMC) of HIV-1-positive immunocompetent and immunocompromised patients can harbour JCV genome, but their precise role in JCV latency or reactivation status before the onset of PML remains hypothetical. Objectives: To assess JCV latency or reactivation status in PBMC of HIV-1-positive immunocompromised patients without PML. Design: A group of 82 HIV-1-positive immunocompromised patients who did not have PML were compared with 10 patients with AIDS and PML and with 69 HIV-1-positive immunocompetent patients without PML. Methods: DNA and total RNA were extracted from PBMC. The presence of ICV DNA was demonstrated by a semi-nested polymerase chain reaction (PCR). By using primer pairs specific for an early gene, T, and a late gene, VP1, the expression of both early and late gene mRNA in PBMC could be identified using reverse transcriptase (RT) PCR. Results: JCV DNA was detected by PCR in 17.4% of 69 HIV-1-positive immunocompetent patients, in 23.2% of 82 HIV-1-positive immunocompromised patients, and in 60% of 10 patients with AIDS and PML. No correlation could be drawn between the detection of ICV DNA in the PBMC and the clinical or biological status of the HIV-1-positive patients. By using RT-PCR procedures, no expression of ICV early and late mRNA in PBMC was found in any patients. Conclusions: JCV DNA is detectable in the PBMC of 20.5% of 151 HIV-1-infected patients independently of the CDC (Centers for Disease Control and Prevention) stages of the infection. Moreover, our results suggest that active replication of JCV in PBMC appears to be absent or at least a very rare event in HIV-1-positive immunocompromised patients with and without PML. (C) 1999 Lippincott Williams & Wilkins.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 18:37:53