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Titolo:
Immunoblot analysis of a 10 kDa antigen in cyst fluid of Taenia solium metacestodes
Autore:
Yang, HJ; Chung, JY; Yun, DH; Kong, Y; Ito, A; Ma, L; Liu, YH; Lee, SC; Kang, SY; Cho, SY;
Indirizzi:
Sung Kyun Kwan Univ, Coll Med, Dept Mol Parasitol, Suwon 440746, South Korea Sung Kyun Kwan Univ Suwon South Korea 440746 , Suwon 440746, South Korea Korea Adv Inst Sci & Technol, Biomed Res Ctr, Seoul 136791, South Korea Korea Adv Inst Sci & Technol Seoul South Korea 136791 36791, South Korea Catholic Univ Korea, Sch Med, Dept Parasitol, Seoul 137701, South Korea Catholic Univ Korea Seoul South Korea 137701 , Seoul 137701, South Korea Gifu Univ, Sch Med, Dept Parasitol, Gifu 500, Japan Gifu Univ Gifu Japan500 Univ, Sch Med, Dept Parasitol, Gifu 500, Japan Chongqing Univ, Affiliated Hosp 1, Inst Infect & Parasit Dis, Chongqing 630044, Peoples R China Chongqing Univ Chongqing Peoples R China 630044 630044, Peoples R China Chung Ang Univ, Coll Med, Dept Parasitol, Seoul 156756, South Korea Chung Ang Univ Seoul South Korea 156756 sitol, Seoul 156756, South Korea
Titolo Testata:
PARASITE IMMUNOLOGY
fascicolo: 10, volume: 20, anno: 1998,
pagine: 483 - 488
SICI:
0141-9838(199810)20:10<483:IAOA1K>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
LINKED IMMUNOELECTROTRANSFER BLOT; ECHINOCOCCUS-GRANULOSUS; BOVINE CYSTICERCOSIS; VESICULAR FLUID; GLYCOPROTEIN ANTIGENS; NEUROCYSTICERCOSIS; ELISA; DISEASE; IMMUNODIAGNOSIS; ANTIBODIES;
Keywords:
Taenia solium; cysticercosis; alveolar echinococcosis; cystic echinococcosis; serodiagnosis; cyst fluid antigen;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Cho, SY Sung Kyun Kwan Univ, Coll Med, Dept Mol Parasitol, Suwon 440746, South Korea Sung Kyun Kwan Univ Suwon South Korea 440746 440746, South Korea
Citazione:
H.J. Yang et al., "Immunoblot analysis of a 10 kDa antigen in cyst fluid of Taenia solium metacestodes", PARASITE IM, 20(10), 1998, pp. 483-488

Abstract

The diagnostic value of a 10 kDa subunit of 150 kDa protein in cyst fluid (CF) of Taenia solium metacestodes was evaluated Immunoblot analysis revealed that most ser a from patients with neurocysticercosis recognized the 10 kDa subunit strongly (209/217 cases, 84.6%), while a few sera from individuals with other parasitic diseases including alveolar echinococcosis (AE, 2/20, 10%) and cystic echinococcosis (CE, 2/25, 8%) showed faint reactions, Sera of cases with other parasitic diseases, especially AE and CE, exhibitedcross reactions against other bands in CF, Both differential immunoblot and immunoprecipitation analyses showed that the 10 kDa subunit was the most specific to cysticercosis and highly antigenic, whereas other components at20-40, 64, 95 and 106 kDa in CF were cross-reactive. IgG subclass ELISA and immunoblot demonstrated that both IgG4 and IgC1 reactions were predominant in neurocysticercosis and recognized the 10 kDa.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/20 alle ore 10:29:21