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Titolo:
Biochemical characterization and localization of Fasciola hepatica 26-28 kDa diagnostic coproantigen
Autore:
Abdel-Rahman, S; OReilly, KL; Malone, JB;
Indirizzi:
Louisiana State Univ, Sch Vet Med, Dept Vet Microbiol & Parasitol, Baton Rouge, LA 70803 USA Louisiana State Univ Baton Rouge LA USA 70803 , Baton Rouge, LA 70803 USA
Titolo Testata:
PARASITE IMMUNOLOGY
fascicolo: 6, volume: 21, anno: 1999,
pagine: 279 - 286
SICI:
0141-9838(199906)21:6<279:BCALOF>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
LINKED-IMMUNOSORBENT-ASSAY; EXCRETORY-SECRETORY ANTIGENS; CIRCULATING ANODIC ANTIGEN; SCHISTOSOMA-MANSONI; MONOCLONAL-ANTIBODIES; CATHODIC ANTIGEN; CATTLE; INDIVIDUALS; PROTEINS;
Keywords:
Fasciola hepatica; trematode; coproantigen; excretory-secretory product (ES); diagnosis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: O'Reilly, KL Assiut Univ, Fac Med, Dept Parasitol, Assiut, Egypt Assiut Univ Assiut Egypt ed, Dept Parasitol, Assiut, Egypt
Citazione:
S. Abdel-Rahman et al., "Biochemical characterization and localization of Fasciola hepatica 26-28 kDa diagnostic coproantigen", PARASITE IM, 21(6), 1999, pp. 279-286

Abstract

We have previously reported the usefulness of a 26-28 kDa coproantigen of Fasciola hepatica for diagnosis of infection, In this study, the 26-28 kDa coproantigen was biochemically characterized with the aid of monoclonal antibodies (MoAb) in an effort to better understand the biology of the antigen. Differential staining of chromatographically-purified 26-28 kDa coproantigen on SDS-PAGE, under reducing and nan-reducing conditions indicated that the coproantigen was a monomeric, highly glycosylated glycoprotein. Alkaline treatment of the purified coproantigen resulted in an 8 kDa protein core which still contained the epitope recognized by the MoAb. No protease activity was associated with the 26-28 kDa coproantigen. The coproantigen could be cleaved by trypsin without altering the reactive epitope recognized by the MoAb, but was resistant to pepsin digestion. Further, the coproantigen was stable under several different storage conditions. Indirect immunofluorescence on tissue sections of adult flukes indicated that the coproantigen was present in gut cells and tegument, Taken together these results confirm the stability of the 26-28 kDa coproantigen and its usefulness in diagnostic tests for F. hepatica infections.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 16/07/20 alle ore 06:23:12