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Titolo:
TRANSCRIPTIONAL REPRESSION OF THE GROWTH-ASSOCIATED T-ALPHA-1 ALPHA-TUBULIN GENE BY TARGET CONTACT
Autore:
WU WD; GLOSTER A; MILLER FD;
Indirizzi:
MCGILL UNIV,MONTREAL NEUROL INST,DEPT NEUROL & NEUROSURG,CTR NEURONALSURVIVAL,3801 RUE UNIV MONTREAL PQ H3A 2B4 CANADA MCGILL UNIV,MONTREAL NEUROL INST,DEPT NEUROL & NEUROSURG,CTR NEURONALSURVIVAL MONTREAL PQ H3A 2B4 CANADA
Titolo Testata:
Journal of neuroscience research
fascicolo: 5, volume: 48, anno: 1997,
pagine: 477 - 487
SICI:
0360-4012(1997)48:5<477:TROTGT>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
SUPERIOR CERVICAL-GANGLION; MESSENGER-RNA; SYMPATHETIC NEURONS; TRANSGENIC MICE; EXPRESSION; REGENERATION; GAP-43; RAT; PROTEINS; PERIPHERIN;
Keywords:
MOTOR NEURONS; SYMPATHETIC NEURONS; NEURONAL REGENERATION; AXONAL INJURY; TRANSCRIPTIONAL ANALYSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
W.D. Wu et al., "TRANSCRIPTIONAL REPRESSION OF THE GROWTH-ASSOCIATED T-ALPHA-1 ALPHA-TUBULIN GENE BY TARGET CONTACT", Journal of neuroscience research, 48(5), 1997, pp. 477-487

Abstract

In this report, we address the molecular mechanisms that regulate axonal growth by focusing on the gene for one of the major axonal cytoskeletal proteins, T alpha 1 alpha-tubulin. During the developmental growth of sympathetic neurons, transcription of a beta-galactosidase transgene driven by the T alpha 1 promoter (T alpha 1:nlacZ) was high untilthe time of target innervation and neuronal maturation, when it decreased significantly, In mature animals, T alpha 1:nlacZ transcription remained relatively low until target contact was experimentally disrupted; when facial motoneurons were axotomized, T alpha 1:nlacZ transgeneexpression increased, was maximal for 1-7 days, and, if neurons regenerated and reinnervated their target musculature, returned to control levels by 49 days, In contrast, if regeneration and reestablishment oftarget contact were inhibited, transgene expression remained elevated, To determine whether this increased transcription was due to the loss of target contact or to axonal loss, we transected sympathetic neurons that project to the eye either close to or far from their cell bodies, In both cases, when target contact was severed, T alpha 1:nlacZ transcription increased, These experiments indicate that transcription of the T alpha 1 alpha-tubulin promoter is repressed by target contact in both developing and mature neurons, We suggest that this repressionis due to a target-derived ''stop-growth'' factor that retrogradely signals to regulate transcription of this and other genes that are required for axonal growth, (C) 1997 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 05:01:39