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Titolo:
The Src family tyrosine kinase is involved in Rho-dependent activation of c-Jun N-terminal kinase by G alpha(12)
Autore:
Nagao, M; Kaziro, Y; Itoh, H;
Indirizzi:
Tokyo Inst Technol, Fac Biosci & Biotechnol, Midori Ku, Yokohama, Kanagawa2268501, Japan Tokyo Inst Technol Yokohama Kanagawa Japan 2268501 Kanagawa2268501, Japan
Titolo Testata:
ONCOGENE
fascicolo: 31, volume: 18, anno: 1999,
pagine: 4425 - 4434
SICI:
0950-9232(19990805)18:31<4425:TSFTKI>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
SERUM RESPONSE FACTOR; GTP-BINDING PROTEIN; CELL-SHAPE CHANGES; ALPHA-SUBUNIT; DROSOPHILA GASTRULATION; TRANSFORMING ACTIVITY; SIGNALING PATHWAY; G13 PROTEINS; GENE-PRODUCT; P115 RHOGEF;
Keywords:
G alpha(12); Src; Rho; JNK;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Itoh, H Tokyo Inst Technol, Fac Biosci & Biotechnol, Midori Ku, 4259 Nagatsuta Cho, Yokohama, Kanagawa 2268501, Japan Tokyo Inst Technol 4259 Nagatsuta Cho Yokohama Kanagawa Japan 2268501
Citazione:
M. Nagao et al., "The Src family tyrosine kinase is involved in Rho-dependent activation of c-Jun N-terminal kinase by G alpha(12)", ONCOGENE, 18(31), 1999, pp. 4425-4434

Abstract

G alpha(12), a member of alpha subunit of heterotrimeric G protein G(12) subfamily, has been shown to stimulate c-Jun N-terminal kinase (JNK) activity through the low molecular weight GTP-binding proteins Ras, Rac, and Cdc42. In this study using the transient expression of a constitutively activated mutant of G alpha(12) (G alpha(12)Q229L) in human embryonic kidney (HEK) 293 cells, we found that Rho and Src family kinase are also involved in theGu,,induced activation of JNK. The activation of JNK by G alpha(12)Q229L was inhibited by dominant-negative RhoA(T19N), and botulinum C3 exoenzyme which specifically inactivates Rho. In addition, the expression of activated RhoA(G14V) elevated JNK activity in HEK 293 cells. The G alpha(12)Q229L-stimulated activation of JNK was blocked by a specific inhibitor of protein tyrosine kinases (PP2), and C-terminal Src kinase (Csk). Moreover, we observed that G alpha(12)Q229L stimulated Src family kinase activity and v-Src induced JNK activation. Interestingly, the v-Src-induced activation of JNK wasinhibited by dominant-negative RhoA(T19N). In contrast, Csk did not inhibit the JNK activation by activated RhoA(G14V). These results suggest that Rho and Src family kinase are required for the G alpha(12)-induced JNK activation, and that Src family kinase acts upstream of Rho activation in the JNKpathway.

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Documento generato il 21/01/21 alle ore 04:09:10