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Titolo:
Optical mapping reveals the functional organization of the trigeminal nuclei in the chick embryo
Autore:
Sato, K; Momose-Sato, Y; Mochida, HN; Arai, Y; Yazawa, I; Kamino, K;
Indirizzi:
Tokyo Med & Dent Univ, Sch Med, Dept Physiol, Bunkyo Ku, Tokyo 1138519, Japan Tokyo Med & Dent Univ Tokyo Japan 1138519 unkyo Ku, Tokyo 1138519, Japan
Titolo Testata:
NEUROSCIENCE
fascicolo: 2, volume: 93, anno: 1999,
pagine: 687 - 702
SICI:
0306-4522(1999)93:2<687:OMRTFO>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
VOLTAGE-SENSITIVE DYES; BRAIN-STEM; ELECTRICAL-ACTIVITY; NERVE-TERMINALS; NEURAL ACTIVITY; POSTSYNAPTIC POTENTIALS; VAGAL-STIMULATION; NEURONAL-ACTIVITY; RESPONSES; RAT;
Keywords:
optical mapping; trigeminal nucleus; functional organization; development; synaptic connections; chick embryo;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Momose-Sato, Y Tokyo Med & Dent Univ, Sch Med, Dept Physiol, Bunkyo Ku, 1-5-45 Yushima, Tokyo 1138519, Japan Tokyo Med & Dent Univ 1-5-45 Yushima Tokyo Japan 1138519 n
Citazione:
K. Sato et al., "Optical mapping reveals the functional organization of the trigeminal nuclei in the chick embryo", NEUROSCIENC, 93(2), 1999, pp. 687-702

Abstract

The functional organization of the trigeminal nuclei during embryogenesis was investigated using multiple-site optical recording with a fast voltage-sensitive dye. Brainstem preparations, with three classified trigeminal nerve afferents, the ophthalmic, maxillary and mandibular nerves, together with motor nerve fibers, were dissected from five- to eight-day-old chick embryos. Electrical responses evoked by trigeminal nerve stimulations were optically recorded simultaneously from many loci of the stained preparations. We identified three response areas related to the trigeminal nerve: area I, located cephalic to the level of the trigeminal ganglion; area II, located caudal to the level of the trigeminal ganglion; and area III, located at the level of the trigeminal root. The neural responses in areas I and II wereevoked by ophthalmic, maxillary or mandibular nerve stimulation, while theresponses in area In were detected when the stimulation was applied to thetrigeminal motor nerve. In comparison with the morphology indicated by DiIlabeling, the results suggest that areas I, II and In correspond to the principal sensory nucleus of the trigeminal nerve, the spinal sensory nucleusof the trigeminal nerve and the trigeminal motor nucleus, respectively. Weidentified two components of the optical response: a fast and a slow signal. In five-day-old preparations, fast spike-like signals related to action potentials were recorded from the three response areas. In six-day-old preparations, slow optical signals which reflect glutamate-mediated excitatory postsynaptic potentials were detected from area II, only when the ophthalmic nerve was stimulated: no slow signal was evoked by maxillary or mandibular nerve stimulation In seven- and eight-day-old preparations, slow signals were detected from both areas I and II with every nerve stimulation. These results suggest that synaptic function is first generated in the spinal trigeminal nucleus by the six-day embryonic stage, and the developmental organization of synaptic function is not the same in the three trigeminal nervesor in the two sensory nuclei. Contour line maps of the signal amplitude revealed that the size and the area of the neural responses within the trigeminal nuclei changed dramatically with development. We compared the spatial distribution and temporal dynamics of the optical signals between the ophthalmic, maxillary and mandibular nerve stimulations, and we found that somatotopic organization is less clear in a rostrocaudal/mediolateral X-Y plane,although the areas of the maxillary and mandibular nerves appeared to separate in the lateral direction. (C) 1999 IBRO. Published by Elsevier ScienceLtd.

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Documento generato il 25/01/20 alle ore 18:37:52