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Titolo:
Gene expression analysis by transcript profiling coupled to a gene database query
Autore:
Shimkets, RA; Lowe, DG; Tai, JTN; Sehl, P; Jin, HK; Yang, RH; Predki, PF; Rothberg, BEG; Murtha, MT; Roth, ME; Shenoy, SG; Windemuth, A; Simpson, JW; Simons, JF; Daley, MP; Gold, SA; McKenna, MP; Hillan, K; Went, GT; Rothberg, JM;
Indirizzi:
CuraGen Corp, New Haven, CT 06511 USA CuraGen Corp New Haven CT USA 06511CuraGen Corp, New Haven, CT 06511 USA Genentech Inc, Dept Cardiovasc Res, S San Francisco, CA 94080 USA Genentech Inc S San Francisco CA USA 94080 S San Francisco, CA 94080 USA Genentech Inc, Dept Pathol, S San Francisco, CA 94080 USA Genentech Inc SSan Francisco CA USA 94080 S San Francisco, CA 94080 USA
Titolo Testata:
NATURE BIOTECHNOLOGY
fascicolo: 8, volume: 17, anno: 1999,
pagine: 798 - 803
SICI:
1087-0156(199908)17:8<798:GEABTP>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA EXPRESSION; CARDIAC-HYPERTROPHY; PRESSURE-OVERLOAD; RAT-HEART; DNA MICROARRAY; ADULT-RAT; HYBRIDIZATION; CDNA; SEQUENCE; MUSCLE;
Keywords:
cardiac hypertrophy; differential gene expression; transcript profiling; oligonucleotide poisoning;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Shimkets, RA CuraGen Corp, 555 Long Wharf Dr, New Haven, CT 06511 USA CuraGen Corp 555 Long Wharf Dr New Haven CT USA 06511 511 USA
Citazione:
R.A. Shimkets et al., "Gene expression analysis by transcript profiling coupled to a gene database query", NAT BIOTECH, 17(8), 1999, pp. 798-803

Abstract

We describe an mRNA profiling technique for determining differential gene expression that utilizes, but does not require, prior knowledge of gene sequences, This method permits high-throughput reproducible detection of most expressed sequences with a sensitivity of greater than 1 part in 100,000, Gene identification by database query of a restriction endonuclease fingerprint, confirmed by competitive PCR using gene-specific oligonucleotides, facilitates gene discovery by minimizing isolation procedures. This process, called GeneCalling, was validated by analysis of the gene expression profiles of normal and hypertrophic rat hearts following in vivo pressure overload.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/08/20 alle ore 05:37:24