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Titolo:
Detection of mRNA transcripts of cyclic nucleotide phosphodiesterase subtypes in ejaculated human spermatozoa
Autore:
Richter, W; Dettmer, D; Glander, HJ;
Indirizzi:
Univ Leipzig, Dept Dermatol, Androl Unit, D-04103 Leipzig, Germany Univ Leipzig Leipzig Germany D-04103 drol Unit, D-04103 Leipzig, Germany Univ Leipzig, Dept Biochem, D-04103 Leipzig, Germany Univ Leipzig Leipzig Germany D-04103 t Biochem, D-04103 Leipzig, Germany
Titolo Testata:
MOLECULAR HUMAN REPRODUCTION
fascicolo: 8, volume: 5, anno: 1999,
pagine: 732 - 736
SICI:
1360-9947(199908)5:8<732:DOMTOC>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-TYROSINE PHOSPHORYLATION; HUMAN-SPERM; ACROSOME REACTION; HORMONAL-REGULATION; MESSENGER-RNA; PENTOXIFYLLINE; MOTILITY; CAPACITATION; INHIBITORS; FERTILIZATION;
Keywords:
cyclic nucleotide phosphodiesterase-subtypes; human spermatozoa; polymerase chain reaction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Glander, HJ Univ Leipzig, Dept Dermatol, Androl Unit, Liebigstr 21, D-04103 Leipzig, Germany Univ Leipzig Liebigstr 21 Leipzig Germany D-04103 ig, Germany
Citazione:
W. Richter et al., "Detection of mRNA transcripts of cyclic nucleotide phosphodiesterase subtypes in ejaculated human spermatozoa", MOL HUM REP, 5(8), 1999, pp. 732-736

Abstract

Multiple types and subtypes, including splice variants, of cyclic nucleotide phosphodiesterases (PDE) have been shown to be expressed in various tissues and organs. They control the intracellular level of cyclic nucleotides and are involved in hormonal signalling. In human spermatozoa, PDE play an important role in the regulation of motility, capacitation and acrosome reaction. The aim of this study was to investigate which transcripts of the different PDE types and subtypes could be found in human spermatozoa using reverse transcription-polymerase chain reaction (RT-PCR). Ejaculated spermatozoa from 10 single semen samples as well as another three semen sample pools were separated by swim-up and were investigated by RT-PCR. We obtained PCR products of the PDE types/subtypes 1A/B/C. 2, 3A/B, 4A/B/C, 5, and 8 withdifferent intensities. Control PCR for leukocyte contamination were negative and contamination by other somatic cells was excluded by the spermatozoapreparation protocol, immunohistochemistry and visual examination. These results demonstrated for the first time that human ejaculated spermatozoa contain an extended pattern of PDE mRNA transcripts.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/21 alle ore 15:06:03