Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Leptin directly stimulates aromatase activity in human luteinized granulosa cells
Autore:
Kitawaki, J; Kusuki, I; Koshiba, H; Tsukamoto, K; Honjo, H;
Indirizzi:
Kyoto Prefectural Univ Med, Dept Obstet & Gynecol, Kamigyo Ku, Kyoto 6028566, Japan Kyoto Prefectural Univ Med Kyoto Japan 6028566 Ku, Kyoto 6028566, Japan
Titolo Testata:
MOLECULAR HUMAN REPRODUCTION
fascicolo: 8, volume: 5, anno: 1999,
pagine: 708 - 713
SICI:
1360-9947(199908)5:8<708:LDSAAI>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR-I; MONOCLONAL-ANTIBODY; FEMALE MICE; OBESE GENE; CYTOCHROME-P-450; EXPRESSION; LOCALIZATION; CLONING; WEIGHT; STAT3;
Keywords:
aromatase; cytochrome P450; leptin; luteinized granulosa cells; oestrogen;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Kitawaki, J Kyoto Prefectural Univ Med, Dept Obstet & Gynecol, Kamigyo Ku,465 Kajii Cho, Kyoto 6028566, Japan Kyoto Prefectural Univ Med 465 Kajii Cho Kyoto Japan 6028566
Citazione:
J. Kitawaki et al., "Leptin directly stimulates aromatase activity in human luteinized granulosa cells", MOL HUM REP, 5(8), 1999, pp. 708-713

Abstract

Leptin, the obese (ob) gene product, is secreted by adipocytes and regulates appetite through interaction with hypothalamic leptin receptors. Leptin may also have a stimulatory effect on reproductive function. Furthermore, leptin receptor mRNA is expressed in the ovary, suggesting a direct effect on its function. The present study examines the direct role of leptin on theoestrogen-producing activity in human luteinized granulosa cells. The cells were obtained from in-vitro fertilization pre-ovulatory follicles, precultured for 24 h in the presence of 5% charcoal-treated serum, and incubated for 48-96 h in a serum-free medium containing recombinant human leptin, follicle stimulating hormone (FSH), and/or insulin-like growth factor-I (IGF-I). A single addition of leptin (0.5-10 ng/ml) stimulated aromatase activitywith the incubation time of up to 96 h. The addition of leptin (1 ng/ml) further augmented the stimulation by a single addition of FSH (100 ng/ml) orIGF-I (100 ng/ml), or a combination of both. A single addition of leptin (1 ng/ml) or a combination of leptin (1 ng/ml), FSH (100 ng/ml), and IGF-I (100 ng/ml) gave rise to an increase in each parameter of oestrogen-producing activity measured, i.e. P450arom mRNA level, P450arom protein level, aromatase specific activity, and the oestradiol concentration in the culture supernatant. However, the production of progesterone did not change. These results indicate that leptin stimulates oestrogen production by increasing P450arom mRNA and P450arom protein expression and, consequently, aromatase activity by its direct action on the human luteinized granulosa cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/10/20 alle ore 09:58:05