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Titolo:
Evaluation of AFLP, a high-resolution DNA fingerprinting method, as a toolfor molecular subtyping of enterohemorrhagic Escherichia coli O157 : H7 isolates
Autore:
Lyoda, S; Wada, A; Weller, J; Flood, SJA; Schreiber, E; Tucker, B; Watanabe, H;
Indirizzi:
Natl Inst Infect Dis, Dept Bacteriol, Shinjuku Ku, Tokyo 1628640, Japan Natl Inst Infect Dis Tokyo Japan 1628640 injuku Ku, Tokyo 1628640, Japan Perkin Elmer Appl Biosyst, Foster City, CA 94404 USA Perkin Elmer Appl Biosyst Foster City CA USA 94404 ter City, CA 94404 USA
Titolo Testata:
MICROBIOLOGY AND IMMUNOLOGY
fascicolo: 8, volume: 43, anno: 1999,
pagine: 803 - 806
SICI:
0385-5600(1999)43:8<803:EOAAHD>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
FIELD GEL-ELECTROPHORESIS; STRAINS; JAPAN;
Keywords:
EHEC O157 : H7; DNA subtyping; AFLP; PFGE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
12
Recensione:
Indirizzi per estratti:
Indirizzo: Watanabe, H Natl Inst Infect Dis, Dept Bacteriol, Shinjuku Ku, Toyama 1-23-1, Tokyo 1628640, Japan Natl Inst Infect Dis Toyama 1-23-1 Tokyo Japan 1628640 Japan
Citazione:
S. Lyoda et al., "Evaluation of AFLP, a high-resolution DNA fingerprinting method, as a toolfor molecular subtyping of enterohemorrhagic Escherichia coli O157 : H7 isolates", MICROB IMMU, 43(8), 1999, pp. 803-806

Abstract

The amplified fragment-length polymorphism (AFLP(TM)) technique is based on the selective PCR amplification of restriction fragments. We investigatedthe utility of AFLP in the molecular subtyping of enterohemorrhagic Escherichia coli serotype O157:H7 isolates. We analyzed a total of 46 isolates ofE, coli O157:H7 along with other serotypes, O26:H11, O114:H19 and O119:NT. Isolates of E. coli O157:H7 derived from the same outbreak showed an identical AFLP-banding pattern and were subtyped into the same group, giving results almost consistent with those of a pulsed-held gel electrophoresis (PFGE) study, while other serotypes showed clearly different patterns from those off. coli O157:H7, These results suggest that the AFLP technique has potential as an alternative tool for the molecular epidemiology of E, coli O157:H7.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 04:25:02