Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Vascular permeability effect of adenovirus-mediated vascular endothelial growth factor gene transfer to the rabbit and rat skeletal muscle
Autore:
Poliakova, L; Kovesdi, I; Wang, XT; Capogrossi, MC; Talan, M;
Indirizzi:
NIA, Intramural Res Program, Gerontol Res Ctr, Gene Therapy Unit,Lab Cardiovasc Sci,NIH, Baltimore, MD 21224 USA NIA Baltimore MD USA 21224 ab Cardiovasc Sci,NIH, Baltimore, MD 21224 USA GenVec Inc, Rockville, MD USA GenVec Inc Rockville MD USAGenVec Inc, Rockville, MD USA Ist Dermopatico Immacolata, Lab Patol Vasc, Rome, Italy Ist Dermopatico Immacolata Rome Italy lata, Lab Patol Vasc, Rome, Italy
Titolo Testata:
JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY
fascicolo: 2, volume: 118, anno: 1999,
pagine: 339 - 347
SICI:
0022-5223(199908)118:2<339:VPEOAV>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERIPHERAL ARTERY DISEASE; THERAPEUTIC ANGIOGENESIS; LIMB ISCHEMIA; THERAPIES; VEGF(165); MODEL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Talan, M NIA, Intramural Res Program, Gerontol Res Ctr, Gene Therapy Unit,Lab Cardiovasc Sci,NIH, 5600 Nathan Shock Dr, Baltimore, MD 21224 USA NIA 5600 Nathan Shock Dr Baltimore MD USA 21224 ore, MD 21224 USA
Citazione:
L. Poliakova et al., "Vascular permeability effect of adenovirus-mediated vascular endothelial growth factor gene transfer to the rabbit and rat skeletal muscle", J THOR SURG, 118(2), 1999, pp. 339-347

Abstract

Objective: Vascular endothelial growth factor has been used in preclinicalstudies and phase 1 and 2 clinical trials as a potent mediator of therapeutic angiogenesis; however, its ability to enhance the vascular permeabilitymay be a source of potential complications. The objective of this work wasto evaluate the effects of the intramuscular injection of an adenovirus vector coding for the 121-amino acid form of vascular endothelial growth factor (Ad.VEGF(121)) on vascular permeability and edema development in rabbitsand rats. Methods: Different concentrations of Ad.VEGF(121) ranging from 10(5) to 10(10) plaque-forming units/mL (3 x 10(6)-3 x 10(11) particles/mL) were injected into hind limb or forelimb muscles of Wistar rats or rabbits. The size of the scrotum, the circumferences of limbs, and the concentration of vascular endothelial growth factor in the serum were measured daily after injection. Results: The injection of different concentrations of Ad.VEGF(121) into the hind limb muscles of rabbits led to a dose-dependent scrotal edema in rabbits at concentrations higher than 107 plaque-forming units/mL (P = .002). The edema developed slowly, reached its maximum level 6 days after the injection, and spontaneously resolved thereafter. At concentrations higher than 10(9) plaque-forming units/mL the scrotal edema was accompanied by skin necrosis (P = .0001). No scrotal edema was observed in rats. Conclusions: The massive species-specific scrotal edema accompanied by skin ulceration and necrosis was observed only in rabbits treated with Ad.VEGF(121) in concentrations exceeding therapeutic doses. The therapeutic doses of Ad.VEGF(121) resulted in only moderate transient scrotal edema in rabbits, suggesting that the potential for side effects of vascular endothelial growth factor therapy as a result of increased vascular permeability should notbe very alarming for generally healthy patients and may not cause a significant clinical problem in the treatment of peripheral vascular diseases.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 04:51:18