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Titolo:
Role of phosphate and kinetic characteristics of complete iron release from native pig spleen ferritin-Fe
Autore:
Huang, HQ; Lin, QM; Kong, B; Zeng, RY; Qiao, YH; Chen, CH; Zhang, FZ; Xu, LS;
Indirizzi:
Xiamen Univ, Dept Biol, Div Biotech, Xiamen 361005, Peoples R China XiamenUniv Xiamen Peoples R China 361005 Xiamen 361005, Peoples R China Xiamen Univ, Res Ctr Environm Sci, Xiamen 361005, Peoples R China Xiamen Univ Xiamen Peoples R China 361005 Xiamen 361005, Peoples R China State Ocean Adm, Inst Oceanog 3, Xiamen 361005, Peoples R China State Ocean Adm Xiamen Peoples R China 361005 en 361005, Peoples R China Xiamen Univ, State Tumor Res Lab, Xiamen 361005, Peoples R China Xiamen Univ Xiamen Peoples R China 361005 Xiamen 361005, Peoples R China Natl Univ Singapore, Inst Mol & Cell Biol, Singapore 117609, Singapore Natl Univ Singapore Singapore Singapore 117609 ngapore 117609, Singapore
Titolo Testata:
JOURNAL OF PROTEIN CHEMISTRY
fascicolo: 4, volume: 18, anno: 1999,
pagine: 497 - 504
SICI:
0277-8033(199905)18:4<497:ROPAKC>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
VINELANDII BACTERIAL FERRITIN; AZOTOBACTER-VINELANDII; HORSE SPLEEN; BACTERIOFERRITIN; BINDING; OXIDATION; CORES;
Keywords:
pig spleen ferritin; kinetics and order; phosphate role and channel function; ascorbic acid and dithionite;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Huang, HQ Xiamen Univ, Dept Biol, Div Biotech, Xiamen 361005, Peoples R China Xiamen Univ Xiamen Peoples R China 361005 005, Peoples R China
Citazione:
H.Q. Huang et al., "Role of phosphate and kinetic characteristics of complete iron release from native pig spleen ferritin-Fe", J PROTEIN C, 18(4), 1999, pp. 497-504

Abstract

The kinetics for complete iron release showing biphasic behavior from pig spleen ferritin-Fe (PSFF) was measured by spectrophotometry. The native core within the PSFF shell consisted of 1682 hydroxide Fe3+ and 13 phosphate molecules. Inhibition kinetics for complete iron release was measure by differential spectrophotometry in the presence of phosphate; the process was clearly divided into two phases involving a first-order reaction at an increasing rate of 46.5 Fe3+/PSFF/min on the surface of the iron core and a zero-order reaction at a decreasing rate of 6.67 Fe3+/PSFF/min inside the core. The kinetic equation [C(PSFF-Fe3+)(max) - C(PSFF-Fe3+)(t)](1/2) = T-max -T-t gives the transition time between the two rates and represents the complex kinetic characteristics. The rate was directly accelerated twofold by a mixed reducer of dithionite and ascorbic acid. These results suggest that the channel of the PSFF shell may carry out multiple functions for iron metabolism and storage and that the phosphate strongly affects the rate of iron release.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 05:43:42