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Titolo:
Determination of melatonin in biological samples by capillary electrophoresis
Autore:
Kim, YO; Chung, HJ; Chung, ST; Kim, JH; Park, JH; Han, SY; Kil, KS; Cho, DH;
Indirizzi:
Natl Inst Toxicol Res, Dept Toxicol, Korea FDA, Eunpyung Gu, Seoul 122704,South Korea Natl Inst Toxicol Res Seoul South Korea 122704 Seoul 122704,South Korea
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY A
fascicolo: 1-2, volume: 850, anno: 1999,
pagine: 369 - 374
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; PINEAL MELATONIN; ELECTROCHEMICAL DETECTION; FLUOROMETRIC DETECTION;
Keywords:
melatonin; hormones;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Kim, YO Natl Inst Toxicol Res, Dept Toxicol, Korea FDA, Eunpyung Gu, Seoul122704,South Korea Natl Inst Toxicol Res Seoul South Korea 122704 22704,South Korea
Citazione:
Y.O. Kim et al., "Determination of melatonin in biological samples by capillary electrophoresis", J CHROMAT A, 850(1-2), 1999, pp. 369-374

Abstract

The determination of melatonin (MLT) in physiological samples was investigated using capillary electrophoresis (CE). Mouse blood was collected in tubes containing EDTA, centrifuged at 1500 g for 20 min at 4 degrees C, and stored at -20 degrees C. Plasma samples were extracted with dichloroethane, centrifuged and the aqueous phase was discarded. Then the organic phase was evaporated to dryness. The residue was dissolved in deionized water and filtered with a microfilter (0.22 mu m). Separations were carried out using a CE system equipped with a fused silica capillary [80 cm (effective length 52 cm)X75 mu m I.D.] and an ultraviolet-visible detector (200 nm), and programmed to provide 25 mM 2-(N-morpholino)ethanesulfonic acid (pH 5.7). Injection was performed hydrostatically by elevating the sample by 10 cm at the cathodic side of the capillary. The calibration curve, reproducibility, recovery and limit of detection were examined, and validation of the method wasperformed. The result showed that MLT in blood could be easily determined with the new method. (C) 1999 Elsevier Science B.V. All rights reserved.

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Documento generato il 29/03/20 alle ore 15:39:41