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Titolo:
Asymmetry of the spindle pole bodies and spg1p GAP segregation during mitosis in fission yeast
Autore:
Cerutti, L; Simanis, V;
Indirizzi:
ISREC, Cell Cycle Control Lab, CH-1068 Epalinges, Switzerland ISREC Epalinges Switzerland CH-1068 Lab, CH-1068 Epalinges, Switzerland
Titolo Testata:
JOURNAL OF CELL SCIENCE
fascicolo: 14, volume: 112, anno: 1999,
pagine: 2313 - 2321
SICI:
0021-9533(199907)112:14<2313:AOTSPB>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-DIVISION CYCLE; DOSAGE-DEPENDENT REGULATOR; SCHIZOSACCHAROMYCES-POMBE; SEPTUM FORMATION; PROTEIN-KINASE; S-POMBE; MONOCLONAL-ANTIBODIES; BIPOLAR SPINDLE; MITOTIC CONTROL; F-ACTIN;
Keywords:
mitosis; cytokinesis; spindle pole body; Schizosaccharomyces pombe;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Simanis, V ISREC, Cell Cycle Control Lab, Chemin Boveresses 155, CH-1068 Epalinges, Switzerland ISREC Chemin Boveresses 155 Epalinges Switzerland CH-1068 land
Citazione:
L. Cerutti e V. Simanis, "Asymmetry of the spindle pole bodies and spg1p GAP segregation during mitosis in fission yeast", J CELL SCI, 112(14), 1999, pp. 2313-2321

Abstract

In the fission yeast Schizosaccharomyces pombe, the onset of septum formation is induced by a signal transduction network involving several protein kinases and a GTPase switch. One of the roles of the spg1p GTPase is to localise the cdc7p protein kinase to the poles of the mitotic spindle, from where the onset of septation is thought to be signalled at the end of mitosis. Immunofluorescence studies have shown that cdc7p is located on both spindle pole bodies early in mitosis, but only on one during the later stages of anaphase, This is mediated by inactivation of spg1p on one pole before the other. The GAP for spg1p is a complex of two proteins, cdc16p and byr4p. Localisation of cdc16p and byr4p hy indirect immunofluorescence during the mitotic cell cycle showed that both proteins are present on the spindle pole body in interphase cells. During mitosis, byr4p is seen first on both polesof the spindle, then on only one. This occurs prior to cdc7p becoming asymmetric. In contrast, the signal due to cdc16p decreases to a low level during early mitosis, before being seen strongly on the same pole as byr4p, Double staining indicates that this is the opposite pole to that which retainscdc7p in late anaphase, Examination of the effect of inactivating cdc16p at various stages of the cell cycle suggests that cdc16p, together with cdc2p plays a role in restraining septum formation during interphase, The asymmetric inactivation of spg1p is mediated by recruitment of the cdc16p-byr4p GAP to one of the poles of the spindle before the other, and the asymmetry of the spindle pole bodies may be established early during mitosis, Moreover, the spindle pole bodies appear to be non-equivalent even after division has been completed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 00:06:27