Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Involvement of fibronectin in the regulation of urokinase production and binding in murine mammary tumor cells
Autore:
Urtreger, AJ; Ghiso, JAA; Werbajh, SE; Puricelli, LI; Muro, AF; Joffe, EBD;
Indirizzi:
Univ Buenos Aires, Inst Oncol Angel H Roffo, Res Area, RA-1417 Buenos Aires, DF, Argentina Univ Buenos Aires Buenos Aires DF Argentina RA-1417 Aires, DF, Argentina Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Biol, Mol Biol & Physiol Lab, Buenos Aires, DF, Argentina Univ Buenos Aires Buenos Aires DF Argentina Buenos Aires, DF, Argentina Int Ctr Genet Engn & Biotechnol, I-34012 Trieste, Italy Int Ctr Genet Engn& Biotechnol Trieste Italy I-34012 012 Trieste, Italy
Titolo Testata:
INTERNATIONAL JOURNAL OF CANCER
fascicolo: 5, volume: 82, anno: 1999,
pagine: 748 - 753
SICI:
0020-7136(19990827)82:5<748:IOFITR>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMINOGEN-ACTIVATOR PRODUCTION; FIBROSARCOMA CELLS; MESSENGER-RNA; RECEPTOR; EXPRESSION; LINES; ADENOCARCINOMAS; MODULATION; COMPONENTS; INTEGRINS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Joffe, EBD Univ Buenos Aires, Inst Oncol Angel H Roffo, Res Area, Av San Martin 5481,RA-1417 Buenos Aires, DF, Argentina Univ Buenos Aires Av San Martin 5481 Buenos Aires DF Argentina RA-1417
Citazione:
A.J. Urtreger et al., "Involvement of fibronectin in the regulation of urokinase production and binding in murine mammary tumor cells", INT J CANC, 82(5), 1999, pp. 748-753

Abstract

Tumor invasion and metastasis development is a multistep process involvingadhesion molecules as well as tumor proteases, It has been reported that tumor cells lacking fibronectin (FN) expression and engineered to re-expressFN showed a marked reduction in metastatic ability. Besides its effects oncell adhesion and migration, FN could be modulating other cellular events associated with the metastatic cascade. To test this hypothesis, we analyzed the production of urokinase-type plasminogen activator (uPA), and its receptor (uPAR), 2 molecules involved in the invasive phenotype, in cells overexpressing RGD wild-type FN (FNwt clones) or RGD-mutated FN (FN RGD-minus clones). Secreted uPA activity and antigen were significantly up-regulated in FN-expressing clones, although RGD-minus cells secreted approximately 50%less uPA than the FNwt ones. Interestingly, while control and FN RGD-minusclones were able to readily bind uPA to their surface, FNwt clones exhibited impaired uPA binding, Furthermore, treatment of the parental cell line as well as the control and FN-expressing clones with exogenous purified FN or RGD peptides induced up-regulation of uPA production and the reduction ofuPA membrane binding, which was associated with lower expression of uPAR. This modulation by FN was found to be dependent on RGD sequence and PI integrin, These results strongly suggest a novel activity for the multifunctional glycoprotein FN regarding the regulation of uPA production as well as the capacity of tumor cells to bind uPA, Int. J, Cancer 82:748-753, 1999, (C)1999 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 10:08:46