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Titolo:
Peptide specificity, HLA class II restriction, and T-cell subsets of the T-cell clones specific to either Cry j 1 or Cry j 2, the major allergens of Japanese cedar (Cryptomeria japonica) pollen
Autore:
Sone, T; Morikubo, K; Shimizu, K; Komiyama, N; Tsunoo, H; Kino, K;
Indirizzi:
Saitama Med Sch, Dept Med Zool, Moroyama, Saitama 3500495, Japan Saitama Med Sch Moroyama Saitama Japan 3500495 ma, Saitama 3500495, Japan Meiji Inst Hlth Sci, Pharmaceut Res Dept, Kanagawa, Japan Meiji Inst Hlth Sci Kanagawa Japan Pharmaceut Res Dept, Kanagawa, Japan
Titolo Testata:
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY
fascicolo: 3, volume: 119, anno: 1999,
pagine: 185 - 196
SICI:
1018-2438(199907)119:3<185:PSHCIR>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERLEUKIN-4 PRODUCTION; SUBCUTANEOUS INJECTION; LYMPHOKINE PRODUCTION; IMMUNE-RESPONSE; ATOPIC PATIENTS; IN-VIVO; EPITOPES; ANTIGEN; INDIVIDUALS; IDENTIFICATION;
Keywords:
Cry j 1; Cry j 2; T-cell clone; HLA class II restriction; T-cell epitopes; Th1/Th2;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Sone, T Saitama Med Sch, Dept Med Zool, 38 Morohongo, Moroyama, Saitama 3500495, Japan Saitama Med Sch 38 Morohongo Moroyama Saitama Japan 3500495 Japan
Citazione:
T. Sone et al., "Peptide specificity, HLA class II restriction, and T-cell subsets of the T-cell clones specific to either Cry j 1 or Cry j 2, the major allergens of Japanese cedar (Cryptomeria japonica) pollen", INT A AL IM, 119(3), 1999, pp. 185-196

Abstract

Background: Cry j 1 and Cry j 2 are thought to be the major allergens of Japanese cedar pollen. HLA class II types capable of presenting T-cell epitopes in both allergens and their role in induction of T-cell subsets are notwell known. Methods: CD4+ T (Th)-cell clones (TCCs) specific to either Cryj 1 or Cry j 2 were generated. HLA class II restrictions were determined by their reactivity to the T-cell epitope in the presence of antigen presenting cells sharing matched types. Interleukin (IL)-2, interferon-gamma, IL-4, and IL-5 contents in the supernatants of TCCs were estimated using enzymeimmunoassay. Results: Peripheral blood mononuclear cells (PBMC) from patients induced proliferation with 100 mu g/ml Cry j 1 or 3-10 mu g/ml rCry j 2stimulation. T-cell epitopes in Cry j 1 were presented to Th cells by the gene products of DRA1*01/DRB1*0901, DRA1*01/DRB5*0101, DQA1*0102/DQB1*0602,and DPA1*01/DPB1*0501; those in Cry j 2 were restricted by DRA1*01/DRB1*0901, DRA1*01/DRB1*1501, DRA1*01/DRB4*01, DRA1*01/DRB5*0101, DQA1*0102/DQB1*0602, DPA1*01/DPB1*0201, and DPA1*01 and *0202/DPB1*0501. Type 2-like cells were preferentially induced in Cry j 1 stimulation, while an almost equal number of type 2- and type 1-like cells was induced in rCry j 2. Conclusions: No clear correlation existed between peptide specificity, HLA class II restriction and induction of Th-cell subsets, suggesting that the requirementof different dose of Cry j 1 or Cry j 2 to induce proliferation in PBMC may lead to distinguishable difference in induction of Th subsets between TCCs specific to Cry j 1 and Cry j 2.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 08:47:36