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Titolo:
Intracellular ice formation is affected by cell interactions
Autore:
Acker, JP; Larese, A; Yang, H; Petrenko, A; McGann, LE;
Indirizzi:
Univ Alberta, Dept Lab Med & Pathol, Edmonton, AB T6G 2R8, Canada Univ Alberta Edmonton AB Canada T6G 2R8 hol, Edmonton, AB T6G 2R8, Canada Canadian Blood Serv, Edmonton, AB, Canada Canadian Blood Serv Edmonton ABCanada Blood Serv, Edmonton, AB, Canada Lab Cair LGL, Lyon, France Lab Cair LGL Lyon FranceLab Cair LGL, Lyon, France Ukrainian Acad Sci, Inst Problems Cryobiol & Cryomed, UA-310108 Kharkov, Ukraine Ukrainian Acad Sci Kharkov Ukraine UA-310108 UA-310108 Kharkov, Ukraine
Titolo Testata:
CRYOBIOLOGY
fascicolo: 4, volume: 38, anno: 1999,
pagine: 363 - 371
SICI:
0011-2240(199906)38:4<363:IIFIAB>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
CRYOPRESERVATION; HEPATOCYTES; SURVIVAL; TISSUES;
Keywords:
intracellular ice formation; cell interactions; cell adhesion; tissue cryobiology; cryomicroscopy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Acker, JP Univ Alberta, Dept Lab Med & Pathol, Edmonton, AB T6G 2R8, Canada Univ Alberta Edmonton AB Canada T6G 2R8 ton, AB T6G 2R8, Canada
Citazione:
J.P. Acker et al., "Intracellular ice formation is affected by cell interactions", CRYOBIOLOGY, 38(4), 1999, pp. 363-371

Abstract

Cell-to-cell and cell-to-surface interactions are important to the structure and function of tissues. These interactions are also important determinants of low-temperature responses in tissues. Four in vitro models using hamster fibroblast cells in tissue culture were used to investigate the influence of cell-cell and cell-surface interactions on intracellular ice formation in these systems. The four models were: (a) single cells in suspension; (b) cells individually attached to glass with only cell-to-surface adhesion; (c) colonies of cells attached to glass with both cell-cell and cell-surface interactions; and (d) multicellular spheroids with extensive cell-cell contacts. Cryomicroscopy was used to monitor the prevalence and kinetics ofintracellular ice formation after ice nucleation in the extracellular solution. The temperature for intracellular freezing in 50% of the cells was significantly affected by both cell-cell and cell-surface interactions. Therewas also evidence of intercellular nucleation through cell-cell interactions. The results indicate that cell-cell and cell-surface interactions play a significant role in the low-temperature response of tissue systems. (C) 1999 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 16:30:15