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Titolo:
Permeation of the luminal capillary glycocalyx is determined by hyaluronan
Autore:
Henry, CBS; Duling, BR;
Indirizzi:
Univ Virginia, Hlth Sci Ctr, Dept Mol Physiol & Biol Phys, Charlottesville, VA 22906 USA Univ Virginia Charlottesville VA USA 22906 Charlottesville, VA 22906 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
fascicolo: 2, volume: 46, anno: 1999,
pagine: H508 - H514
SICI:
0363-6135(199908)46:2<H508:POTLCG>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOTHELIAL-CELL GLYCOCALYX; PERICELLULAR MATRIX; SURFACE; ACID; BINDING; PERMEABILITY; RECEPTOR; INVITRO; RAT;
Keywords:
hamster cremaster muscle; hyaluronidase; endothelial cell; fluorescence microscopy; fiber matrix;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Duling, BR Univ Virginia, Hlth Sci Ctr, Dept Mol Physiol & Biol Phys, POB 10011, Charlottesville, VA 22906 USA Univ Virginia POB 10011 Charlottesville VA USA 22906 22906 USA
Citazione:
C.B.S. Henry e B.R. Duling, "Permeation of the luminal capillary glycocalyx is determined by hyaluronan", AM J P-HEAR, 46(2), 1999, pp. H508-H514

Abstract

The endothelial cell glycocalyx influences blood flow and presents a selective barrier to movement of macromolecules from plasma to the endothelial surface. In the hamster cremaster microcirculation, FITC-labeled Dextran 70 and larger molecules are excluded from a region extending almost 0.5 mu m from the endothelial surface into the lumen. Red blood cells under normal now conditions are excluded from a region extending even farther into the lumen. Examination of cultured endothelial cells has shown that the glycocalyxcontains hyaluronan, a glycosaminoglycan which is known to create matriceswith molecular sieving properties. To test the hypothesis that hyaluronan might be involved in establishing the permeation properties of the apical surface glycocalyx in vivo, hamster microvessels in the cremaster muscle were visualized using video microscopy. After infusion of one of several FITC-dextrans (70, 145, 580, and 2,000 kDa) via a femoral cannula, microvessels were observed with bright-field and fluorescence microscopy to obtain estimates of the anatomic diameters and the widths of fluorescent dextran columns and of red blood cell columns (means +/- SE). The widths of the red bloodcell and dextran exclusion zones were calculated as one-half the difference between the bright-field anatomic diameter and the width of the red bloodcell column or dextran column. After 1 h of treatment with active Streptomyces hyaluronidase, there was a significant increase in access of 70- and 145-kDa FITC-dextrans to the space bounded by the apical glycocalyx, but no increase in access of the red blood cells or in the anatomic diameter in capillaries, arterioles, and venules. Hyaluronidase had no effect on access of FITC-Dextrans 580 and 2,000. Infusion of a mixture of hyaluronan and chondroitin sulfate after enzyme treatment reconstituted the glycocalyx, although treatment with either molecule separately had no effect. These results suggest that cell surface hyaluronan plays a role in regulating or establishing permeation of the apical glycocalyx to macromolecules. This finding andour prior observations suggest that hyaluronan and other glycoconjugates are required for assembly of the matrix on the endothelial surface. We hypothesize that hyaluronidase creates a more open matrix, enabling smaller dextran molecules to penetrate deeper into the glycocalyx.

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Documento generato il 21/09/20 alle ore 19:15:37