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Titolo:
HIV-1 subtype C syncytium- and non-syncytium-inducing phenotypes and coreceptor usage among Ethiopian patients with AIDS
Autore:
Abebe, A; Demissie, D; Goudsmit, J; Brouwer, M; Kuiken, CL; Pollakis, G; Schuitemaker, H; Fontanet, AL; de Wit, TFR;
Indirizzi:
Ethiopian Hlth & Nutr Res Inst, Ethiopian Netherlands AIDS Res Project, Addis Ababa, Ethiopia Ethiopian Hlth & Nutr Res Inst Addis Ababa Ethiopia dis Ababa, Ethiopia Univ Addis Ababa, Fac Nat Sci, Dept Biol, Addis Ababa, Ethiopia Univ AddisAbaba Addis Ababa Ethiopia Dept Biol, Addis Ababa, Ethiopia Univ Amsterdam, Acad Med Ctr, Dept Human Retrovirol, NL-1105 AZ Amsterdam,Netherlands Univ Amsterdam Amsterdam Netherlands NL-1105 AZ AZ Amsterdam,Netherlands Sanquin Blood Supply Fdn, Dept Clin Viral Immunol, CLB, Amsterdam, Netherlands Sanquin Blood Supply Fdn Amsterdam Netherlands , Amsterdam, Netherlands Univ Calif Los Alamos Natl Lab, Los Alamos, NM USA Univ Calif Los Alamos Natl Lab Los Alamos NM USA Lab, Los Alamos, NM USA
Titolo Testata:
AIDS
fascicolo: 11, volume: 13, anno: 1999,
pagine: 1305 - 1311
SICI:
0269-9370(19990730)13:11<1305:HSCSAN>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; BIOLOGICAL PHENOTYPE; GENOTYPIC CHARACTERISTICS; TYPE-1 INFECTION; VIRAL PHENOTYPE; ADDIS-ABABA; T-CELL; INDIVIDUALS; CAPACITY; CCR5;
Keywords:
HIV-1; C-subtype; AIDS; SI; NSI; coreceptor; Ethiopia;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Abebe, A Ethiopian Hlth & Nutr Res Inst, Ethiopian Netherlands AIDS Res Project, POB 1242, Addis Ababa, Ethiopia Ethiopian Hlth & Nutr Res Inst POB 1242 Addis Ababa Ethiopia ia
Citazione:
A. Abebe et al., "HIV-1 subtype C syncytium- and non-syncytium-inducing phenotypes and coreceptor usage among Ethiopian patients with AIDS", AIDS, 13(11), 1999, pp. 1305-1311

Abstract

Objective: To assess syncytium-inducing (SI) and non-syncytium-inducing (NSI) frequencies, coreceptor usage and gp120 V3 sequences of HIV-1 isolates from Ethiopian AIDS patients. Patients: Cross-sectional study on 48 hospitalized AIDS patients (CD4 T cells < 200 x 10(6) cell/l) with stage III or IV of the WHO staging system for HIV-1 infection and disease. Methods: Peripheral blood mononuclear cells (PBMC) from all 48 patients were tested by MT-2 assay to determine SI/NSI phenotypes. Lymphocyte subsets were enumerated using Coulter counting and FACScan analysis. Viral load determination used a nucleic acid sequence-based amplification assay (NASBA). Coreceptor usage of HIV-1 biological clones was measured using U87 CD4/chemokine receptor transfectants and phytohemaggrutinin-stimulated PBMC of healthy donors with wildtype CCR5 and homozygous mutation CCR5 Delta 32 (a 32 base-pair deletion in CCR5). Reverse transcriptase polymerase chain reactionsequencing was performed on the third variable region (V3) of the HIV-1 gene gp120. Sequence alignments were done manually; phylogenetic analyses used PHYLIP software packages. Results: SI viruses were detected for 3/48 (6%) AIDS patients only. Lower mean absolute CD4 counts were determined in patients with SI virus comparedwith NSI (P = 0.04), but no differences in viral load were observed. All patients were found to be infected with HIV-1 subtype C, based on V3 sequencing. NSI biological clones used CCR5 as coreceptor; SI biological clones used CXCR4 and/or CCR5 and/or CCR3. Conclusions: Ethiopian patients with HIV-1 C-subtype AIDS harbour a remarkably low frequency of SI phenotype viruses. Coreceptor usage of these viruses correlates with their biological phenotypes. (C) 1999 Lippincott Williams & Wilkins.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 05:46:27