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Titolo:
Cerebral 6-[F-18]fluoro-L-DOPA (FDOPA) metabolism in pig studied by positron emission tomography
Autore:
Danielsen, EH; Smith, DF; Gee, AD; Venkatachalam, TK; Hansen, SB; Hermansen, F; Gjedde, A; Cumming, P;
Indirizzi:
Aarhus Univ Hosp, PET Ctr, DK-8000 Aarhus C, Denmark Aarhus Univ Hosp Aarhus Denmark C sp, PET Ctr, DK-8000 Aarhus C, Denmark Aarhus Univ, Hosp Psychiat, Dept Biol Psychiat, DK-8240 Risskov, Denmark Aarhus Univ Risskov Denmark DK-8240 l Psychiat, DK-8240 Risskov, Denmark McGill Univ, Montreal Neurol Inst, McConnell Brain Imaging Ctr, Montreal, PQ, Canada McGill Univ Montreal PQ Canada l Brain Imaging Ctr, Montreal, PQ, Canada
Titolo Testata:
SYNAPSE
fascicolo: 4, volume: 33, anno: 1999,
pagine: 247 - 258
SICI:
0887-4476(19990915)33:4<247:C6(MIP>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
CATECHOL-O-METHYLTRANSFERASE; DOPA DECARBOXYLASE ACTIVITY; LIVING HUMAN BRAIN; PARKINSONS-DISEASE; IN-VIVO; NONHUMAN-PRIMATES; BLOOD-FLOW; F-18 DOPA; DIFFERENTIAL-DIAGNOSIS; PET;
Keywords:
6-[F-18]fluoro-L-DOPA; FDOPA; dopamine; metabolic rates; positron emission tomography; PET; neuroimaging; swine/pigs/porcine striatum; partial volume;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Danielsen, EH Aarhus Univ Hosp, PET Ctr, Norrebrogade 44, DK-8000 Aarhus C, Denmark Aarhus Univ Hosp Norrebrogade 44 Aarhus Denmark C , Denmark
Citazione:
E.H. Danielsen et al., "Cerebral 6-[F-18]fluoro-L-DOPA (FDOPA) metabolism in pig studied by positron emission tomography", SYNAPSE, 33(4), 1999, pp. 247-258

Abstract

We measured 6-[F-18] fluoro-L-DOPA (FDOPA) uptake and metabolism in the brain of 4-month-old female pigs (n = 8) using a high-resolution positron emission tomograph (PET) in 3D mode. The mean net blood-brain clearance of FDOPA (K-i(D)) to striatum was 0.011 mi g(-1) min(-1). Correcting for the elimination of decarboxylated metabolites from striatum (k(loss) = 0.004 min(-1)) increased the apparent magnitude of the estimate of K-i(D) by 50%, at the expense of doubling the variance of the mean estimate. The mean decarboxylation rate of FDOPA in striatum relative to the cerebellum input (k(3)(s))was 0.008 min(-1). For multicompartmental analyses, the FDOPA partition volume (V-e(D)) was constrained to the individual value observed in cerebellum (mean = 0.53 mi g(-1)), with correction for the presence in brain of the plasma metabolite 3-O-methyl-FDOPA (OMFD). Using the first 60 min of the dynamic PET scans, the rate constant of FDOPA decarboxylation (k(3)(D)) was estimated to be 0.037 min(-1) in striatum, but was not significantly different than zero in frontal cortex. Fitting of a compartmental model correctingfor elimination of decarboxylated metabolites to the complete PET frame-sequence (120 min) increased the variance of the estimate of k(3)(D) in striatum. The magnitude of k(3)(D) in striatum of young pig was less than valuesestimated previously in neonatal piglet, adult monkey, and human. MRI-based simulations predicted that recovery of radioactivity from pig striatum was highly sensitive to the volume of interest. We conclude that the spatial resolution of our tomograph reduces the apparent magnitude of k(3)(D) in striatum. However, anaesthetised pigs are an appropriate experimental model for PET studies of DOPA decarboxylation in striatum. Synapse 33:247-258, 1999, (C) 1999 Wiley-Liss, Inc.

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Documento generato il 29/10/20 alle ore 21:40:57