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Titolo:
Differences in osteopontin up-regulation between proximal and distal tubules after renal ischemia/reperfusion
Autore:
Persy, VP; Verstrepen, WA; Ysebaert, DK; De Greef, KE; De Broe, ME;
Indirizzi:
Univ Antwerp, Dept Nephrol, B-2020 Antwerp, Belgium Univ Antwerp AntwerpBelgium B-2020 ept Nephrol, B-2020 Antwerp, Belgium Univ Antwerp, Dept Expt Surg, B-2020 Antwerp, Belgium Univ Antwerp Antwerp Belgium B-2020 t Expt Surg, B-2020 Antwerp, Belgium
Titolo Testata:
KIDNEY INTERNATIONAL
fascicolo: 2, volume: 56, anno: 1999,
pagine: 601 - 611
SICI:
0085-2538(199908)56:2<601:DIOUBP>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT-KIDNEY CELLS; PHYSIOLOGICAL-PROPERTIES; NONPHOSPHORYLATED FORMS; CRYSTAL-GROWTH; POTENTIAL ROLE; IN-VITRO; EXPRESSION; FAILURE; INJURY; INHIBITION;
Keywords:
acute renal failure; ischemia; regeneration; necrosis; phosphoprotein; cell adhesion; renal injury;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: De Broe, ME Univ Antwerp Hosp, Dept Nephrol, Wilrijkstr 10, B-2650 Edegem,Belgium Univ Antwerp Hosp Wilrijkstr 10 Edegem Belgium B-2650 Belgium
Citazione:
V.P. Persy et al., "Differences in osteopontin up-regulation between proximal and distal tubules after renal ischemia/reperfusion", KIDNEY INT, 56(2), 1999, pp. 601-611

Abstract

Background. Osteopontin (OPN) is a highly acidic phosphoprotein containingan arginine-glycine-aspartic acid (RGD) cell adhesion motif. High OPN expression has been found in tissues with high cell turnover, and OPN up-regulation has been demonstrated in several models of renal injury, suggesting a possible role in tissue remodeling and repair. However, its exact function in the kidney remains unknown. In this study, the possible contribution of OPN to regeneration and repair in the kidney was explored by studying the time course and subcellular localization of OPN up-regulation after renal ischemia/reperfusion injury in different nephron segments and by investigating its relationship with tubular morphology. Methods. Rats that underwent 60 minutes of left renal ischemia and a rightnephrectomy sacrificed at 10 different time points (from 1 hr to 10 days after reperfusion) were compared with uninephrectomized rats at each time point. In renal tissue sections immunostained for OPN, proximal (PTs) and distal tubules (DTs) in both the renal cortex and outer stripe of the outer medulla (OSOM) were scored for the degree of OPN expression and tubular morphology. Results. Kidneys of uninephrectomized rats showed no injury, and the localization and intensity of their OPN expression remained unaltered compared with normal rats. After ischemia/reperfusion, morphological damage was most severe in PTs of the OSOM, but all examined nephron segments showed a significant increase in OPN expression. The time course of OPN up-regulation wasdifferent in PTs and DTs. DTs in both cortex and OSOM rapidly increased their OPN expression, with a maximum at 24 hours after reperfusion followed by a slow decrease. In contrast, PTs showed a delayed increase in OPN staining, with a maximum after five to seven days, higher in the OSOM than in thecortex. In OSOM PTs, OPN expression was predominantly associated with morphological regeneration, whereas DTs showed a substantial OPN up-regulation without major morphological damage. PTs and DTs displayed a different subcellular OPN staining pattern: OPN staining in DTs was located to the apical side of the cell; PTs, however, presented a vesicular, perinuclear stainingpattern. Conclusions. Our study found a different pattern of OPN up-regulation after renal ischemia/reperfusion in PTs versus DTs, both with regard to time course and subcellular localization. DTs show an early and persistent increase in OPN staining in the absence of major morphological injury, whereas OPNstaining in PTs is delayed and is mostly associated with morphological regeneration. PTs show a vesicular, perinuclear OPN staining pattern, whereas DTs show OPN staining at the apical cell side.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 19:45:25