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Titolo:
Expression and branch-specific export of mRNA are regulated by synapse formation and interaction with specific postsynaptic targets
Autore:
Schacher, S; Wu, F; Panyko, JD; Sun, ZY; Wang, DN;
Indirizzi:
Columbia Univ Coll Phys & Surg, Ctr Neurobiol & Behav, New York, NY 10032 USA Columbia Univ Coll Phys & Surg New York NY USA 10032 w York, NY 10032 USA Columbia Univ Coll Phys & Surg, Genome Ctr, New York, NY 10032 USA Columbia Univ Coll Phys & Surg New York NY USA 10032 w York, NY 10032 USA New York State Psychiat Inst, New York, NY 10032 USA New York State Psychiat Inst New York NY USA 10032 New York, NY 10032 USA
Titolo Testata:
JOURNAL OF NEUROSCIENCE
fascicolo: 15, volume: 19, anno: 1999,
pagine: 6338 - 6347
SICI:
0270-6474(19990801)19:15<6338:EABEOM>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
APLYSIA SENSORY NEURONS; TERM HETEROSYNAPTIC FACILITATION; DEPENDENT STRUCTURAL-CHANGES; MESSENGER-RNA LOCALIZATION; DISSOCIATED CELL-CULTURE; LOCAL PROTEIN-SYNTHESIS; IDENTIFIED NEURONS; NEURITE OUTGROWTH; AXONAL-TRANSPORT; IN-VITRO;
Keywords:
synapse formation; specificity; gene expression; mRNA export; RT-PCR; cell culture; Aplysia;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Schacher, S Columbia Univ Coll Phys & Surg, Ctr Neurobiol & Behav, 722 W 168th St, NewYork, NY 10032 USA Columbia Univ Coll Phys & Surg 722 W 168th St New York NY USA 10032
Citazione:
S. Schacher et al., "Expression and branch-specific export of mRNA are regulated by synapse formation and interaction with specific postsynaptic targets", J NEUROSC, 19(15), 1999, pp. 6338-6347

Abstract

Mechanosensory neurons (SNs) of Aplysia form synapses in culture with sometargets (L7), but not others (L11), even when a SN is plated with both targets. We examined whether branch-specific net export of mRNA encoding synapse-specific molecules might contribute to branch-specific synapse formation. Single-cell RT-PCR was used to assay levels of mRNA encoding the SN-specific neuropeptide (sensorin A) and other transcripts in cell bodies and neuritic processes of SNs cultured alone or with synaptic targets. Some mRNAs are exported to neurites, but not others. Sensorin A mRNA is detected only in SN cell bodies and neurites, and expression levels correlate with the strength of the synaptic connections formed with L7 after 4 d in culture. After 4 d, move sensorin A transcripts are detected in SN neurites contacting L7 than in SN neurites contacting L11. The differential expression at 4 d isfound even when a single SN contacts both targets simultaneously. By contrast, no significant difference in expression is detected in SN neurites contacting L7 versus L11 after 1 d of coculture. The results suggest that interaction and synapse formation with a specific target lead to a time-dependent change in the branch-specific accumulation of sensorin A mRNA in SNs. Because local protein synthesis at synaptic sites might contribute to synaptic function or plasticity, the results suggest that branch-specific targeting of mRNA encoding synapse-related molecules may contribute to the formation of specific synapses.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 00:17:24