Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Expression of functional growth hormone secretagogue receptors in human pituitary adenomas: Polymerase chain reaction, triple in-situ hybridization and cell culture studies
Autore:
Barlier, A; Zamora, AJ; Grino, M; Gunz, G; Pellegrini-Bouiller, I; Morange-Ramos, I; Figarella-Branger, D; Dufour, H; Jaquet, P; Enjalbert, A;
Indirizzi:
Univ Mediterranee, Lab Interact Cellulaires Neuroendocriniennes, CNRS,UMR 6544, Inst Jean Roche,Fac Med Nord, F-13916 Marseille 20, France Univ Mediterranee Marseille France 20 Nord, F-13916 Marseille 20, France Fac Med Nord, Lab Neuroendocrinol Expt, INSERM, U501,Inst Federatif Jean Roche, Marseille, France Fac Med Nord Marseille France t Federatif Jean Roche, Marseille, France CHU Timone, Serv Endocrinol, Marseille, France CHU Timone Marseille France Timone, Serv Endocrinol, Marseille, France CHU Timone, Lab Anat Pathol & Neuropathol, Marseille, France CHU Timone Marseille France nat Pathol & Neuropathol, Marseille, France CHU Timone, Serv Neurochirurg, Marseille, France CHU Timone Marseille France imone, Serv Neurochirurg, Marseille, France
Titolo Testata:
JOURNAL OF NEUROENDOCRINOLOGY
fascicolo: 7, volume: 11, anno: 1999,
pagine: 491 - 502
SICI:
0953-8194(199907)11:7<491:EOFGHS>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RIBONUCLEIC-ACID; GH-RELEASING HORMONE; CUSHINGS-SYNDROME; RAT PITUITARY; NORMAL MEN; PEPTIDE; SECRETION; TUMORS; IDENTIFICATION; INFUSION;
Keywords:
GHRP; MK-0677; GHS-receptors; pituitary adenoma; in-situ hybridization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Barlier, A Univ Mediterranee, Lab Interact Cellulaires Neuroendocriniennes, CNRS,UMR 6544, Inst Jean Roche,Fac Med Nord, Bd P Dramard, F-13916 Marseille 20, France Univ Mediterranee Bd P Dramard Marseille France 20 20, France
Citazione:
A. Barlier et al., "Expression of functional growth hormone secretagogue receptors in human pituitary adenomas: Polymerase chain reaction, triple in-situ hybridization and cell culture studies", J NEUROENDO, 11(7), 1999, pp. 491-502

Abstract

We examined the expression of functional growth hormone secretagogue receptors (GHS-R) in a series of 30 human pituitary adenomas-six secreting GH, three GH-PRL, six prolactin (PRL), five adrenocorticotrophic hormone (ACTH),one thyroid stimulating hormone (TSH), four gonadotroph and five non-secreting adenomas. By reverse transcriptase polymerase chain reaction (RT-PCR),the coexpression of the two GHS-R isoforms (la and Ib) was found in all the GH-, GH-PRL- and PRL-secreting adenomas, and only in two out of three corticotroph, two out of four gonadotroph and one out of five non-secreting tumours, They were absent in the TSH-secreting adenoma, The PCR products of GHS-R la and Ib were identical in size to those from two normal pituitaries,PGR cloning and sequencing of isoforms performed in two somatotroph adenomas revealed only two single, silent base mutations. Triple in-situ hybridization showed colocalization of GHS-R mRNA with messengers of GH and PRL, conjointly or separately, in individual cells of somatotroph, mammosomatotroph, and lactotroph adenomas. The presence of GHS-R mRNA in cells expressing PRL mRNA is emphasized. In cultured cells from six somatotroph and two mammosomatotroph adenomas, the powerful GHS MK-0677 stimulated GH release in a dose-dependent manner, with maximal effect at 6 h, Contrarily, when GHRH was applied, only three somatotrophs and two mamosomatotrophs were stimulated. In the two mammosomatotrophs, the PRL response to MK-0677 and to GHRH wassimilar to the GH response. An homologous desensitization of the GHS-R andthe GHRH receptor was observed 24 h after a first stimulation by a single dose of the corresponding agonist, Heterologous desensitization was not observed. Interestingly, MK-0677 also stimulated, in a dose-dependent way, thehormone release of cells from all tested lactotroph and corticotroph adenomas, The existence of a functional expression of GHS-R in somatotroph, mammosomatotroph, lactotroph and corticotroph adenomas rises the question of the role played by GHS-R in pituitary adenomas, particularly those not engaged in GH secretion.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 04:12:15