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Titolo:
Salt effects in capillary zone electrophoresis - IV. Resolution versus time and the effect of potassium phosphate and its concentration in the high ionic strength separation of sulphonamides
Autore:
Cross, RF; Cao, J;
Indirizzi:
Swinburne Univ Technol, Sch Sci & Engn, Hawthorn, Vic 3122, Australia Swinburne Univ Technol Hawthorn Vic Australia 3122 n, Vic 3122, Australia
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY A
fascicolo: 2, volume: 849, anno: 1999,
pagine: 575 - 585
Fonte:
ISI
Lingua:
ENG
Soggetto:
DIHYDROFOLATE-REDUCTASE INHIBITORS; MIGRATION BEHAVIOR; INFLUENCE MOBILITY; SODIUM-PHOSPHATE; SULFONAMIDES; BUFFER; OPTIMIZATION; SELECTIVITY; ELECTROLYTE; PH;
Keywords:
salt effects; resolution; buffer composition; sulphonamides;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Cross, RF Swinburne Univ Technol, Sch Sci & Engn, John St, Hawthorn, Vic 3122, Australia Swinburne Univ Technol John St Hawthorn Vic Australia 3122 ralia
Citazione:
R.F. Cross e J. Cao, "Salt effects in capillary zone electrophoresis - IV. Resolution versus time and the effect of potassium phosphate and its concentration in the high ionic strength separation of sulphonamides", J CHROMAT A, 849(2), 1999, pp. 575-585

Abstract

The effects of potassium phosphate buffer and its concentration upon the capillary zone electrophoretic separation of 23 sulphonamides and a neutral marker were examined at pH 7. The resolution between the pairs was improvedwith the increased concentration of the buffer from 65 mM to 174 mM. Nineteen sulphonamides, a hydrolysis product and several unidentified minor components were baseline resolved in both 101 and 138 mM phosphate buffers. In 174 mM buffer all 21 ionised sulphonamides and the other compounds were separated. A simple relationship between the resolution of analyte pairs (R-s)and the square root of the mean analysis time for the pair (root<(t(app))over bar>) was derived, but few of the pairs displayed this behaviour. For the majority of pairs of compounds, Joule heating appeared to cause a maximum in the R-s versus root<(t(app))over bar> relationship, while non-idealityand shifts in ionisation with increasing salt concentration appeared dominant in other cases. (C) 1999 Elsevier Science B.V. All rights reserved.

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Documento generato il 01/10/20 alle ore 16:08:11