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Titolo:
Multicenter evaluation study on a new HEp2 ANA screening enzyme immune assay
Autore:
Bayer, PM; Bauerfeind, S; Bienvenu, J; Fabien, N; Frei, PC; Gilburd, B; Heide, KG; Hoier-Madsen, M; Meroni, PL; Monier, JC; Monneret, G; Panzeri, P; Shoenfeld, Y; Spertini, F; Wiik, A;
Indirizzi:
Zent Lab Wilhelminenspital, Vienna, Austria Zent Lab Wilhelminenspital Vienna Austria lminenspital, Vienna, Austria Rheumaklin Bad Bramstedt GmbH, Bad Bramstedt, Germany Rheumaklin Bad Bramstedt GmbH Bad Bramstedt Germany Bramstedt, Germany Ctr Hosp, Immunol Lab, Lyon Sud, France Ctr Hosp Lyon Sud FranceCtr Hosp, Immunol Lab, Lyon Sud, France CHU Vaudois, Div Immunol & Allergie, CH-1011 Lausanne, Switzerland CHU Vaudois Lausanne Switzerland CH-1011 , CH-1011 Lausanne, Switzerland Chaim Sheba Med Ctr, Autoimmune Dis Res Unit, IL-52621 Tel Hashomer, Israel Chaim Sheba Med Ctr Tel Hashomer Israel IL-52621 21 Tel Hashomer, Israel Statens Serum Inst, Dept Autoimmunol, Div Diagnost, DK-2300 Copenhagen, Denmark Statens Serum Inst Copenhagen Denmark DK-2300 K-2300 Copenhagen, Denmark IRCCS Policlin, Dipartimento Med Interna, Milan, Italy IRCCS Policlin Milan Italy clin, Dipartimento Med Interna, Milan, Italy
Titolo Testata:
JOURNAL OF AUTOIMMUNITY
fascicolo: 1, volume: 13, anno: 1999,
pagine: 89 - 93
SICI:
0896-8411(199908)13:1<89:MESOAN>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
REVISED CRITERIA; CLASSIFICATION; DISEASES;
Keywords:
antinuclear antibodies; enzyme immunoassay; systemic autoimmune diseases;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
12
Recensione:
Indirizzi per estratti:
Indirizzo: Meroni, PL Univ Milan, Padiglione Granelli IRCCS Policlin, Dept Internal Med, Via F Sforza 35, I-20122 Milan, Italy Univ Milan Via F Sforza 35 MilanItaly I-20122 22 Milan, Italy
Citazione:
P.M. Bayer et al., "Multicenter evaluation study on a new HEp2 ANA screening enzyme immune assay", J AUTOIMMUN, 13(1), 1999, pp. 89-93

Abstract

The COBAS(R) Core HEp2 ANA enzyme immune assay (EIA) was evaluated in a precision and a clinical sample study in comparison to indirect immunofluorescence assay (IFA) on HEp2-cells. In the precision study the COBAS(R) Core EIA yielded intraassay coefficient variations (CVs) mostly below 9%, and interassay CVs between 4.7% and 10.4%. When comparing the COBAS(R) Core EIA toIFA, the results corresponded well in healthy subjects, systemic lupus erythematosus, mixed connective tissue disease and rheumatoid arthritis. In the case of Sjogren's syndrome and scleroderma patients the COBAS(R) Core EIAyielded a lower rate of positive results compared to IFA. This discrepancymay be explained by the lack of detection of autoantibodies to nuclear antigens that can be identified only by IFA due to their compartimentalizationand higher localized antigen density in HEp2 cells. The discrepancies in the group of dermato/polymyositis patients are due to the fact that the EIA contains mainly nuclear antigens and was able to detect only antibodies against the cytoplasmic Jo1 antigen that was added to the HEp2 nuclear extract. Routine sera were also evaluated; good agreement was found in sera from patients attending tertiary reference centres for autoimmune diseases but a higher number of discrepancies was reported in sera from unselected populations. (C) 1999 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 03:50:14